Haloplanus salinus sp. nov., an extremely halophilic archaeon from a Chinese marine solar saltern
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Original Paper
Haloplanus salinus sp. nov., an extremely halophilic archaeon from a Chinese marine solar saltern Xing–Xing Qiu · Mei‑Lin Zhao · Dong Han · Wen‑Jiao Zhang · Heng‑Lin Cui
Received: 3 July 2013 / Revised: 27 September 2013 / Accepted: 29 September 2013 / Published online: 16 October 2013 © Springer-Verlag Berlin Heidelberg 2013
Abstract Halophilic archaeal strain YGH66T was isolated from the Yinggehai marine solar saltern near the Sanya city of Hainan Province, China. Cells were pleomorphic, flat, stained Gram-negative, and produced pinkpigmented colonies. Strain YGH66T was able to grow at 20–50 °C (optimum 37 °C), at 0.9–4.8 M NaCl (optimum 3.1 M NaCl), at 0.005–1.0 M MgCl2 (optimum 0.05 M MgCl2), and at pH 6.0–8.0 (optimum pH 7.0). The cells of strain YGH66T were lysed in distilled water, and the minimum NaCl concentration that prevented cell lysis was 5 % (w/v). The major polar lipids of the strain were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate, one major glycolipid (GL1) chromatographically identical to sulfated mannosyl glucosyl diether and a minor unidentified lipid (GL2), respectively. On the basis of 16S rRNA gene sequence analysis, strain YGH66T was closely related to Haloplanus natans JCM 14081T, Haloplanus aerogenes TBN37T, and Haloplanus vescus RO5-8T with the similarities of 98.0, 97.6, and Communicated by Erko Stackebrandt. The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene and the rpoB′ gene sequences of strain YGH66T are JQ237126 and KF286653, respectively. Phase-contrast micrograph of strain YGH66T, thin-layer chromatograms of strain YGH66T, and related members are available as supplementary materials. Electronic supplementary material The online version of this article (doi:10.1007/s00203-013-0929-z) contains supplementary material, which is available to authorized users. X. Qiu · M.-L. Zhao · D. Han · W.-J. Zhang · H.-L. Cui (*) School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Jingkou District, Zhenjiang 212013, People’s Republic of China e-mail: [email protected]
96.9 %, respectively. The rpoB′ gene similarity between strain YGH66T and the current three members of Haloplanus were 90.4–92.8 %. The DNA G+C content of strain YGH66T was 67.2 mol %. The DNA–DNA hybridization values between strain YGH66T and three members of Haloplanus, H. natans JCM 14081T, H. aerogenes TBN37T, H. vescus RO5-8T were 50, 46 and 39 %, respectively. It was concluded that strain YGH66T represents a novel species of the genus Haloplanus, for which the name Haloplanus salinus sp. nov. is proposed. The type strain is YGH66T (=CGMCC 1.12127T = JCM 18368T). Keywords Haloplanus salinus sp. nov · Halophilic archaeon · Marine solar saltern
Introduction The genus Haloplanus of the family Halobacteriaceae was established by Elevi Bardavid et al. (2007) and currently contains 3 species. Haloplanus natans was described based on three flat-shaped strains isolated from an experimental mesocosm filled with a mixture o
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