Halorubrum rutilum sp. nov. isolated from a marine solar saltern
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ORIGINAL PAPER
Halorubrum rutilum sp. nov. isolated from a marine solar saltern Shuai Yin1 · Zhao Wang1 · Jia‑Qi Xu1 · Wen‑Mei Xu1 · Pan‑Pan Yuan1 · Heng‑Lin Cui1
Received: 30 July 2015 / Revised: 10 September 2015 / Accepted: 28 September 2015 / Published online: 5 October 2015 © Springer-Verlag Berlin Heidelberg 2015
Abstract A halophilic archaeal strain, YJ-18-S1T, was isolated from Yangjiang marine solar saltern, Guangxi Province, China. Cells were pleomorphic, stained Gramnegative and formed red-pigmented colonies on agar plates. Strain YJ-18-S1T was able to grow at 20–55 °C (optimum 37 °C), at 0.9–4.8 M NaCl (optimum 2.6 M NaCl), at 0.005–1.0 M MgCl2 (optimum 0.3 MgCl2) and at pH 5.5–8.5 (optimum pH 7.0). The cells were lysed in distilled water, and the minimal NaCl concentration to prevent cell lysis was found to be 5 % (w/v). The major polar lipids of the strain were phosphatidic acid, phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate and sulfated mannosyl glucosyl diether. The 16S rRNA gene and rpoB′ gene of strain YJ-18-S1T were phylogenetically related to the corresponding genes of Halorubrum members (94.3–98.0 and 86.7–96.1 % similarities, respectively). The DNA G+C content of strain YJ-18-S1T was 66.2 mol%. The phenotypic, chemotaxonomic and phylogenetic properties suggested that strain YJ18-S1T (=CGMCC 1.12554T = JCM 30030T) represents a new species of Halorubrum, for which the name Halorubrum rutilum sp. nov. is proposed.
Communicated by Erko Stackebrandt. Electronic supplementary material The online version of this article (doi:10.1007/s00203-015-1159-3) contains supplementary material, which is available to authorized users. * Heng‑Lin Cui [email protected] 1
School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Jingkou District, Zhenjiang 212013, People’s Republic of China
Keywords Halorubrum rutilum sp. nov. · Halophilic archaeon · Marine solar saltern
Introduction Diverse halophilic archaea, members of the class Halobacteria, are always found to be present in marine solar salterns (Ghai et al. 2011; Narasingarao et al. 2012; Oren 2014). The past several years have witnessed the description of novel species and genera based on the haloarchaeal strains isolated from these kinds of hypersaline environments, such as Halobellus inordinatus (Qiu et al. 2013a), Natronomonas gomsonensis (Kim et al. 2013), Halobacterium rubrum (Han and Cui 2014), Haloarchaeobius litoreus (Zhang and Cui 2014b), Salinigranum rubrum (Cui and Zhang 2014) and Halovenus salina (InfanteDomínguez et al. 2015). During our survey on halophilic archaeal diversity of the Yangjiang marine solar saltern (Guangxi Province, China), we obtained a halophilic archaeal isolate, YJ-18-S1T, that was most closely related to the members of Halorubrum, as judged from 16S rRNA gene sequences. The genus Halorubrum, belonging to the family Haloferacaceae (Gupta et al. 2015), was first established by McGenity and Grant (1995) and currently contains 30 named specie
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