Erythrobacter alti sp. nov., a marine alphaproteobacterium isolated from seawater
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ORIGINAL PAPER
Erythrobacter alti sp. nov., a marine alphaproteobacterium isolated from seawater Jaewoo Yoon1
Received: 12 January 2017 / Revised: 25 April 2017 / Accepted: 28 April 2017 © Springer-Verlag Berlin Heidelberg 2017
Abstract A polyphasic taxonomic study was performed on strain KMU-34T, which was isolated from seawater in the Republic of Korea. The bacterial cells were Gram-stainnegative, strictly aerobic, chemoheterotrophic, yellow-pigmented and rod-shaped. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that the novel marine strain was affiliated with the family Erythrobacteraceae of the class Alphaproteobacteria and that it showed highest sequence similarity (98.6%) to Erythrobacter atlanticus s21-N3T. The DNA–DNA relatedness values between strains KMU-34T and E. atlanticus KCTC 42697T were 8.6 ± 1.2%. The DNA G + C content of strain KMU-34T was determined to be 60.4 mol%. Ubiquinone 10 (Q-10) was the sole respiratory quinone. The predominant cellular fatty acids were C18:1 ω7c (43.8%) and C16:1 ω7c (16.8%). Strain KMU-34T had phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, a sphingoglycolipid, an unidentified phospholipid and two unidentified lipids as polar lipids. From the distinct phylogenetic position and combination of genotypic and phenotypic characteristics, the strain is considered to represent a novel species of the genus Erythrobacter for which the name Erythrobacter alti sp. nov. is proposed. The type strain of
Communicated by Erko Stackebrandt. Electronic supplementary material The online version of this article (doi:10.1007/s00203-017-1384-z) contains supplementary material, which is available to authorized users. * Jaewoo Yoon [email protected] 1
College of Pharmacy, Keimyung University, 1095 Dalgubeoldaero, Dalseo‑Gu, Daegu 42601, Republic of Korea
E. alti sp. nov. is KMU-34T (= KCCM 9 0261T = NBRC 111903T). Keywords Alphaproteobacteria · Erythrobacteraceae · Erythrobacter alti sp. nov. · Seawater · 16S rRNA gene · Polyphasic taxonomy
Introduction The class Alphaproteobacteria (Stackebrandt et al. 1988; Garrity et al. 2005) comprising heterogeneous and diverse phylogenetic groups is considered to represent a major portion of marine microorganisms (Giovannoni and Rappé 2000). The family Erythrobacteraceae of the class was first established by Lee et al. (2005) based on a comprehensive phylogenetic analysis using the 16S rRNA gene sequences. The description of this family was subsequently amended by Xu et al. (2009). At the time of writing, the family comprises five validly published genera: Erythrobacter (Shiba and Simidu 1982), Porphyrobacter (Fuerst et al. 1993), Erythromicrobium (Yurkov et al. 1994), Altererythrobacter (Kwon et al. 2007) and Croceicoccus (Xu et al. 2009). Among them, currently, the genus Erythrobacter includes seventeen species (http://www.bacterio.net/erythrobacteraceae.html) which were isolated from various aquatic and terrestrial environments such as seawater (Denner et al. 2002; Yoon et al. 2004; Jung et
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