Halarcobacter arenosus sp. nov., isolated from marine sediment
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ORIGINAL PAPER
Halarcobacter arenosus sp. nov., isolated from marine sediment Jihye Baek1 · Jiseon Jeong1 · Jong‑Hwa Kim1 · Ampaitip Sukhoom2 · Wonyong Kim1 Received: 5 March 2020 / Revised: 1 September 2020 / Accepted: 1 October 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract A Gram-stain-negative, slightly curved, rod-shaped bacterial strain CAU 1 517T was isolated from marine sediment in Busan, the Republic of Korea. The taxonomic position of strain CAU 1 517T was investigated via a polyphasic approach comprising phenotypic, chemotaxonomic and phylogenetic properties. Strain CAU 1 517T grew optimally at 30 °C, pH 7.5 and in the presence of 7% (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequencing revealed that strain CAU 1517T belongs to the genus Halarcobacter and is most closely related to Halarcobacter bivalviorum LMG 2 6154T (96.5% similarity). The average nucleotide identity and digital DNA-DNA hybridization values between strain CAU 1517T and members of genus Halarcobacter ranged from were 76.7–78.0% and 19.5–21.2%, respectively. The strain contained menaquinone-6 (MK-6) as the only respiratory quinone, and C 16:0, summed feature 3 ( C16:1 ω7c/C16:1 ω6c), and summed feature 8 ( C18:1ω7c/ C18:1ω6c) as the major fatty acids. The polar lipid pattern consisted of diphosphatidylglycerol, phosphatidylethanolamine, and two unidentified aminophospholipids. The G+C content was 28.2 mol%. Therefore, it has been demonstrated that the isolate represents a novel species of the genus Halarcobacter, for which the name Halarcobacter arenosus sp. nov., is proposed. The type strain is CAU 1517T (=KCTC 72232T =NBRC 113955T). Keywords Halarcobacter arenosus · Campylobacteraceae · Sea sediment · Taxonomy
Introduction
The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain CAU 1517T is MK280766 and the Whole Genome Shotgun sequencing project accession number is VANU00000000.
Halarcobacter bivalviorum (Levican et al. 2012), Halarcobacter ebronensis (Levican et al. 2015), and Halarcobacter anaerophilus (Jyothsna et al. 2013) with validly published names (https: //lpsn.dsmz.de/genus/ halarc obact er). Members of the genus Halarcobacter are facultative anaerobic, Gramstin-negative rod-shaped bacteria that are commonly found in marine environments. Halarcobacter species have been isolated from shellfish (Levican et al. 2012), marine (Levican et al. 2015), and an estuarine sediment (Jyothsna et al. 2013). During the screening of novel bacteria from marine sediment sample collected in Busan, the Republic of Korea, a bacterial isolates, designated CAU 1 517T, showing the closest 16S rRNA gene sequence similarities to species of the genus Halarcobacter, is described in this study.
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00203-020-02075-8) contains supplementary material, which is available to authorized users.
Materials and methods
The genus Halarcobacter, a member of the family Campylo
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