Hematological indices and activity of NTPDase and cholinesterase enzymes in rats exposed to cadmium and treated with N -
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Hematological indices and activity of NTPDase and cholinesterase enzymes in rats exposed to cadmium and treated with N-acetylcysteine Jamile F. Gonc¸alves • Marta M. M. F. Duarte • Amanda M. Fiorenza • Roselia M. Spanevello • Cinthia M. Mazzanti • Roberta Schmatz • Margarete D. Bagatini • Fabiane G. Antes • Pauline Costa • Fa´tima H. Abdalla Valderi L. Dressler • Vera M. Morsch • Maria Rosa C. Schetinger
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Received: 20 May 2012 / Accepted: 3 September 2012 / Published online: 19 September 2012 Ó Springer Science+Business Media, LLC. 2012
Abstract The present study aimed to investigate the influence of N-acetylcysteine (NAC) on cadmium (Cd) poisoning by evaluating Cd concentration in tissues, hematological indices as well as the activity of J. F. Gonc¸alves (&) M. R. C. Schetinger (&) Departamento de Bioquı´mica, Instituto de Cieˆncias Ba´sicas da Sau´de, Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcellos 2600-Anexo, Porto Alegre, RS 90035-003, Brazil e-mail: [email protected] M. R. C. Schetinger e-mail: [email protected] M. M. M. F. Duarte A. M. Fiorenza R. Schmatz F. G. Antes P. Costa F. H. Abdalla V. L. Dressler V. M. Morsch M. R. C. Schetinger Departamento de Quı´mica, Centro de Cieˆncias Naturais e Exatas, Universidade Federal de Santa Maria, Campus Universita´rio Camobi, Santa Maria, RS 97105-900, Brazil R. M. Spanevello Centro de Cieˆncias Quı´micas, Farmaceˆuticas e de Alimentos, Setor de Bioquı´mica, Universidade Federal de Pelotas, Campus Universita´rio Capa˜o do Lea˜o, Pelotas, RS 96010-900, Brazil C. M. Mazzanti Departamento de Clı´nica de Pequenos Animais, Setor de Patologia Clı´nica Veterina´ria, Universidade Federal de Santa Maria, Campus Universita´rio, Camobi, Santa Maria, RS 97105-900, Brazil M. D. Bagatini Colegiado do Curso de Enfermagem, Universidade Federal da Fronteira Sul, Chapeco´, SC 89812-000, Brazil
NTPDase, acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) enzymes of rats exposed to Cd and co-treated with NAC. For this purpose, the rats received Cd (2 mg/kg) and NAC (150 mg/kg) by gavage every other day for 30 days. Animals were divided into four groups (n = 6–8): control/saline, NAC, Cd, and Cd/NAC. Cd exposure increased Cd concentration in plasma, spleen and thymus, and NAC co-treatment modulated this augment in both lymphoid organs. Cd exposure reduced red blood cell count, hemoglobin content and hematocrit value. Cd intoxication caused a decrease in total white blood cell count. NAC treatment per se caused an increase in lymphocyte and a decrease in neutrophil counts. On contrary, Cd exposure caused a decrease in lymphocyte and an increase in neutrophil and monocyte counts. NAC reversed or ameliorated the hematological impairments caused by Cd poisoning. There were no significant alterations in the NTPDase activity in lymphocytes of rats treated with Cd and/or NAC. Cd caused a decrease in the activities of lymphocyte AChE, whole blood AChE and serum BChE. However, NAC co-treatment was inefficient in counteracting the negativ
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