Heterologous Protein Production in CHO Cells Methods and Protocols
This volume discusses protocols that cover genetic manipulation of Chinese hamster ovary (CHO) cells for recombinant protein production, and protocols for the characterization of CHO cells using ‘omic approaches. This book also explores methods that discu
- PDF / 6,182,851 Bytes
- 256 Pages / 504.63 x 737.01 pts Page_size
- 13 Downloads / 196 Views
Paula Meleady Editor
Heterologous Protein Production in CHO Cells Methods and Protocols
Methods
in
Molecular Biology
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Heterologous Protein Production in CHO Cells Methods and Protocols
Edited by
Paula Meleady National Institute for Cellular Biotechnology, Dublin City University, Dublin, Ireland
Editor Paula Meleady National Institute for Cellular Biotechnology Dublin City University Dublin, Ireland
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-6971-5 ISBN 978-1-4939-6972-2 (eBook) DOI 10.1007/978-1-4939-6972-2 Library of Congress Control Number: 2017935545 © Springer Science+Business Media LLC 2017 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Cover Illustration: The front cover image, kindly provided by Alan Costello (National Institute for Cellular Biotechnology, Dublin City University), shows Chinese hamster ovary (CHO) cells with inducible green fluorescent protein (GFP) expression (from Chapter 6). Printed on acid-free paper This Humana Press imprint is published by Springer Nature The registered company is Springer Science+Business Media LLC The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.
Preface Since their introduction into the market over 20 years ago, biotherapeutics have constituted a large and growing percentage of the total pharmaceutical market, as well as approximately 25% of the R&D pipeline in industry. These biotherapeutics are having a huge global impact on the treatment of challenging and previously untreatable chronic disease. Currently biopharmaceuticals generate global revenues of $163 billion, making up about 20% of the pharma market, and predicted to gro
Data Loading...
