Protein Chromatography Methods and Protocols
A prerequisite for elucidating the structure and function of any protein is the prior purification of that protein. This necessity has led to the development of many purification schemes and chromatographic methods for the isolation of native proteins fro
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Molecular Biology™
Series Editor John M. Walker School of Life Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
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Protein Chromatography Methods and Protocols
Edited by
Dermot Walls and Sinéad T. Loughran School of Biotechnology and National Centre for Sensor Research, Dublin City University, Dublin, Ireland
Editors Dermot Walls, PhD School of Biotechnology and National Centre for Sensor Research Dublin City University Dublin, Ireland [email protected]
Sinéad T. Loughran, PhD School of Biotechnology and National Centre for Sensor Research Dublin City University Dublin, Ireland [email protected]
ISBN 978-1-60761-912-3 e-ISBN 978-1-60761-913-0 DOI 10.1007/978-1-60761-913-0 Springer New York Dordrecht Heidelberg London Library of Congress Control Number: 2010938718 © Springer Science+Business Media, LLC 2011 All rights reserved. This work may not be translated or copied in whole or in part without the written permission of the publisher (Humana Press, c/o Springer Science+Business Media, LLC, 233 Spring Street, New York, NY 10013, USA), except for brief excerpts in connection with reviews or scholarly analysis. Use in connection with any form of information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed is forbidden. The use in this publication of trade names, trademarks, service marks, and similar terms, even if they are not identified as such, is not to be taken as an expression of opinion as to whether or not they are subject to proprietary rights. While the advice and information in this book are believed to be true and accurate at the date of going to press, neither the authors nor the editors nor the publisher can accept any legal responsibility for any errors or omissions that may be made. The publisher makes no warranty, express or implied, with respect to the material contained herein. Printed on acid-free paper Humana Press is part of Springer Science+Business Media (www.springer.com)
Preface Proteins are essential constituents of all organisms, and they participate in virtually every process within cells. These macromolecules are found in roles that are enzymatic, regulatory, structural, and immunological, to name but a few. In order to elucidate the structure and function of any protein, it is first necessary to purify it, and consequently many purification schemes and chromatographic methods for the isolation of native proteins from complex sources have been developed over the years. Every protein has its own particular sequence of amino acids which is determined by the nucleotide sequence of a corresponding gene. The last 30 years or so has witnessed revolutionary changes in experimental biology and specifically in the way that we identify, isolate, and manipulate individual genes and proteins. Thus, the emergence of recombinant DNA technology, genomics, and bioinformatics, in
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