Protein Chromatography Methods and Protocols
This second edition expands on the previous edition with new chapters that are suitable for newcomers, as well as more detailed chapters that cover protein stability and storage, avoiding proteolysis during chromatography, protein quantitation methods inc
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Dermot Walls Sinéad T. Loughran Editors
Protein Chromatography Methods and Protocols Second Edition
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Protein Chromatography Methods and Protocols Second Edition
Edited by
Dermot Walls School of Biotechnology, Dublin City University, Dublin, Ireland
Sinéad T. Loughran Department of Applied Sciences, Dundalk Institute of Technology, Dublin, Ireland
Editors Dermot Walls School of Biotechnology Dublin City University Dublin, Ireland
Sinéad T. Loughran Department of Applied Sciences Dundalk Institute of Technology Dublin, Ireland
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-6410-9 ISBN 978-1-4939-6412-3 (eBook) DOI 10.1007/978-1-4939-6412-3 Library of Congress Control Number: 2016949739 © Springer Science+Business Media New York 2017 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Printed on acid-free paper This Humana Press imprint is published by Springer Nature The registered company is Springer Science+Business Media LLC New York
Preface Proteins are essential constituents of all organisms and they participate in virtually every process within cells. These macromolecules are to be found in roles that are enzymatic, regulatory, structural, and immunological to name but a few. In order to elucidate the structure and function of any protein it is first necessary to purify it, and consequently many purification schemes and chromatographic methods for the isolation of native proteins from complex sources have been developed over the years. Every protein has its own particular sequence of amino acids that is determined by the nucleotide sequence of a corresponding gene. The last 40 years or so has witnessed revolutionary changes in experimental biology, and in particular in the way that we identify, isolate, a
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