High-Performance Liquid Chromatographic Method for the Determination of Bisphenol A, Nonylphenol and Octylphenol in Sewa
This research is based on high performance liquid chromatographic method for the determination of bisphenol A, nonylphenol and octylphenol in sewage. Sewage samples use dispersive liquid–liquid extraction-pre column derivatization-High performance liquid
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High-Performance Liquid Chromatographic Method for the Determination of Bisphenol A, Nonylphenol and Octylphenol in Sewage Qingzeng Qian, Xiangke Cao, Qian Wang, Yanhua Cao, Suying Rong and Sujing Yu Abstract This research is based on high performance liquid chromatographic method for the determination of bisphenol A, nonylphenol and octylphenol in sewage. Sewage samples use dispersive liquid-liquid extraction-pre column derivatization-High performance liquid chromatography method for the determination. Dichloromethane is extracting agent. Acetonitrile and water (V:V = 90:10) are mobile phase. Bisphenol A, nonylphenol and octylphenol detect by Inertsil ODS-P column (5 μm, 250 × 4.6 mm) after the separation of fluorescence. Fluorescence excitation wavelength is 227 nm. The emission wavelength is 313 nm. Sample complete in 6 min within the peak. Linear range is 0.1−10 mg/L. In 0.1, 0.5 and 1 mg/L three concentrations of added level of sewage recovery of samples are respectively 94.7–106.8 %. The method is simple, rapid and economic actual samples. It can be used for sewage. Keywords High performance liquid chromatography • Sewage • Bisphenol A • Nonyl phenol • Octyl phenol
Q. Qian (*) · Q. Wang · Y. Cao Central Laboratory for College of Public Health, Hebei United University, Tangshan 063000, China e-mail: [email protected] X. Cao Central Laboratory for College of Life Sciences, Hebei United University, Tangshan, China S. Rong College of Tangshan Vocational and Technical, Tangshan, China S. Yu The Fourth Hospital of Tangshan City, Tangshan, China
W. Du (ed.), Informatics and Management Science I, Lecture Notes in Electrical Engineering 204, DOI: 10.1007/978-1-4471-4802-9_91, © Springer-Verlag London 2013
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91.1 Introduction Nonylphenol (NP), octylphenol (OP) and bisphenol A (BPA) belong to the environmental endocrine disruptors (EES). NP and OP are in the environment of alkyl phenol polyoxyethylene ether degradation products. BPA is the production of polycarbonate and epoxy resins [1, 2]. As a result of alkyl phenol polyoxyethylene ether, polycarbonate and epoxy resins are widely used. The nonylphenol, octylphenol and bisphenol A exists widely in various environments [3, 4]. Studies show that NP and BPA can induce male vitellogenin generation. OP major cause of reduced fertility in males. The estrogenic activity of NP is more than 40 times.BPA estrogenic activity has been widely demonstrated [5]. In order to better assess the material impact on the ecological environment, a rapid, accurate, sensitive and efficient analysis method is particularly important. \At present, nonylphenol, octylphenol and bisphenol A analysis method with gas chromatography—mass spectrometry, liquid chromatography mass spectrometry, capillary electrophoresis, micellar electrokinetic chromatography and high performance liquid chromatography [6]. Involving samples are biological, food, water, toys. GC-MS and HPLC-MS method because of its high sensitivity analysis of estrogen of commonly used method
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