Simultaneous Determination of Bisphenol A, Bisphenol F, 4-Nonylphenol, 4- n -Nonylphenol, and Octylphenol in Grease-Rich
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Simultaneous Determination of Bisphenol A, Bisphenol F, 4-Nonylphenol, 4-n-Nonylphenol, and Octylphenol in Grease-Rich Food by Carb/PSA Solid-Phase Extraction Combined with High-Performance Liquid Chromatography Tandem Mass Spectrometry Zhiming Hao 1 & Yanping Xiao 1 & Lanlan Jiang 2 & Weidong Bai 3 & Wenshan Huang 4 & Lipeng Yuan 1
Received: 5 July 2017 / Accepted: 20 August 2017 # Springer Science+Business Media, LLC 2017
Abstract A solid-phase extraction (SPE) combined with high-performance liquid chromatography–tandem mass spectrometry (HPLC-MS/MS) method was developed to determine five phenols in grease-rich food (i.e., hotpot seasoning). Sample added with isotope internal standards bisphenol A-d4 (BPA-d4) and 4-n-nonylphenol-d4 (4-n-NPd4) was dispersedly dissolved in n-hexane. After centrifugation, the supernatant was purified by Carb/PSA SPE. Then, target analytes were separated on a Waters XBridge C18 column, detected under multiple reaction monitoring (MRM) mode. The developed method was validated in terms of linearity, limit of quantitation (LOQ), recovery, and precision. Results found that linear relations were favorable in the selected concentration range of 0.5–100 μg/L for all phenols, with R2 greater than 0.995. LOQs were between 1.0 and 2.0 μg/kg. The mean recoveries for negative sample at three spiked concentration levels were in the range of 83.5–97.1%, and the intra-day and inter-day precision were calculated between 4.7 and 12.5%. This method is accurate and sensitive and has
* Weidong Bai [email protected]
1
Guangdong Agriculture Industry Business Polytechnic College, Guangzhou, China
2
Guangzhou Quality Supervision and Testing Institute, Guangzhou, China
3
College of Light Industry and Food Sciences, Zhongkai University of Agriculture and Engineering, Guangzhou, Guangdong 510225, China
4
Beijing University of Chemical Technology, Beijing, China
good purifying effect, which can be applied for screening and quantitation of target phenols in grease-rich food. Keywords Phenols . Grease-rich food . Solid-phase extraction . HPLC-MS/MS . Isotope internal standard method
Introduction Bisphenol A (BPA) and alkylphenols (APs; especially nonylphenol (NP) and octylphenol (OP)) issues in foods are of increasing concern because of their endocrine-disrupting properties to human beings (Niu et al. 2011; Yang et al. 2014). It has been reported that BPA is widely used in the production of polycarbonate plastics and epoxy resins and the manufacture of polyester resin intermediates, flame retardants, and hydrogenated BPA (Matozzo et al. 2008). As for NP and OP, they have been reported to be used in the production of detergents, herbicides, and synthetic resin products. In recent years, these phenols have been strictly restricted by many regulations and countries. The Commission Directive 2004/19/EC established the specific migration limit (SML) of 0.6 μg/g in foods or food simulants for BPA (Commission Directive 2004/19/EC, Off. J. Eur. Commun. L71 (2004) 8). In addition, the tolerable daily in
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