Identification of contaminants as members of the Bacillus pumilus group and analysis of contamination sources in the cul
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ORIGINAL ARTICLE
Identification of contaminants as members of the Bacillus pumilus group and analysis of contamination sources in the cultivation of Pleurotus geesteranus mushrooms Na Lu 1 & Wei-Dong Yuan 1 & You-Liang Wu 2 & Ji-Ling Song 1 & Lin Ma 3 & Wei-Ke Wang 1 & Jing Yan 1 & Zai-Ming Chen 4 & Qing Chen 5 Received: 1 April 2020 / Accepted: 15 September 2020 # Società Italiana di Patologia Vegetale (S.I.Pa.V.) 2020
Abstract Oyster mushroom (Pleurotus geesteranus Singer) is grown widely in China withut problems, but occasionally severe bacterial contamination occurred in cultivation bags of Pleurotus geesteranus during high summer temperatures in some companies in the Chunan county of Zhejiang province in 2018, belonging to the so-called Bacillus pumilus group by morphological characteristics, 16S rRNA, GyrB and aroE genes sequencing, fatty acid analysis and MALDI analysis. The inoculation tests confirmed that Bacillus pumilus strains were contaminants causing mycelial disappearance of P. geesteranus. The dual culture tests demonstrated that several strains of B. pumilus were able to inhibit mycelial growth of P. geesteranus. In order to find the sources of contamination the, process of mushroom cultivation was investigated. A 41.0% contamination rate occurred in cultivation bags with sponge plugs, as opposed to a very low contamination rate (
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