Immunocytochemistry A Practical Guide for Biomedical Research
Immunocytochemistry is a microscopic method that uses antibodies to localize proteins in tissue sections and cell cultures. Dr. Burry’s book is the first practical guide to planning, performing, and evaluating immunocytochemical experiments designed for n
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Richard W. Burry
Immunocytochemistry A Practical Guide for Biomedical Research
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Richard W. Burry College of Medicine & Public Health Ohio State University 333 West 10th Avenue Columbus, OH 43210-1239 USA [email protected]
ISBN 978-1-4419-1303-6 e-ISBN 978-1-4419-1304-3 DOI 10.1007/978-1-4419-1304-3 Springer New York Dordrecht Heidelberg London Library of Congress Control Number: 2009938351 © Springer Science+Business Media, LLC 2010 All rights reserved. This work may not be translated or copied in whole or in part without the written permission of the publisher (Springer Science+Business Media, LLC, 233 Spring Street, New York, NY 10013, USA), except for brief excerpts in connection with reviews or scholarly analysis. Use in connection with any form of information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed is forbidden. The use in this publication of trade names, trademarks, service marks, and similar terms, even if they are not identified as such, is not to be taken as an expression of opinion as to whether or not they are subject to proprietary rights. While the advice and information in this book are believed to be true and accurate at the date of going to press, neither the authors nor the editors nor the publisher can accept any legal responsibility for any errors or omissions that may be made. The publisher makes no warranty, express or implied, with respect to the material contained herein. Printed on acid-free paper Springer is part of Springer Science+Business Media (www.springer.com)
To Yvonne, my best friend, my wife, and my technical editor, for her love and unwavering support of this project. And to my parents, for lighting the fire in me as a child by giving me a microscope.
Acknowledgments
Thanks to my many colleagues in the Histochemical Society and at The Ohio State University for their discussions that led to the concept of this book. Specifically, to Elizabeth Unger for reading the manuscript at various stages and whose ideas were invaluable and gave me a different understanding of immunocytochemistry; to Paul Robinson for the insight his years of experience gave; to Amy Tovar for centering my ideas; to John Gensel for help with the case studies; to Vidya Kondadasula for ideas early in the project; Mary Jo Burkhard for editing, and to Georgia Bishop for help with organization. Thanks to Ping Wei and Wenmin Lai for the technical help with sectioning. Special thanks to Yvonne Burry for the hours of reading and editing the manuscript. Thanks to Carol Larimer for her editing expertise. Thanks to Stephanie Jakob of Springer for her encouragement and support of this project.
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Contents
1 Introduction . . . . . . . . . . . . . . . . . . . . What Is Immunocytochemistry? . . . . . . . . . . What Can Immunocytochemistry Tell Us? . . . . . An Outline of the Immunocytochemistry Procedure What Is Included in This Book? . . . . . . . . . .
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