Immunohistochemistry: Basics and Methods
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Igor B. Buchwalow
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Werner Bo¨cker
Immunohistochemistry: Basics and Methods
Prof. Dr. Igor B. Buchwalow Prof. Dr. Werner Bo¨cker Gerhard-Domagk-Institut fu¨r Pathologie Domagkstr. 17 48149 Mu¨nster Germany New addresses: Prof. Dr. Igor B. Buchwalow Institute for Hematopathology Fangdieckstrasse 75a 22547 Hamburg Germany [email protected]; [email protected]
Prof. Dr. Werner Bo¨cker Consultation and Reference Center for Gyneco- and Breast-Pathology Fangdieckstrasse 75a 22547 Hamburg Germany [email protected]; [email protected]
ISBN: 978-3-642-04608-7 e-ISBN: 978-3-642-04609-4 DOI 10.1007/978-3-642-04609-4 Springer Heidelberg Dordrecht London New York Library of Congress Control Number: 2009938017 # Springer-Verlag Berlin Heidelberg 2010 This work is subject to copyright. All rights are reserved, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilm or in any other way, and storage in data banks. Duplication of this publication or parts thereof is permitted only under the provisions of the German Copyright Law of September 9, 1965, in its current version, and permission for use must always be obtained from Springer. Violations are liable to prosecution under the German Copyright Law. The use of general descriptive names, registered names, trademarks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. Cover Illustration: Double immunolabeling of cytokeratins in a duct wall of the human mammary gland, see Chap. 8, Fig. 3 (photo by Igor B. Buchwalow, Gerhard-Domagk-Institut fu¨r Pathologie, Mu¨nster, Germany) Cover design: WMXDesign GmbH, Heidelberg, Germany Printed on acid-free paper Springer is part of Springer Science+Business Media (www.springer.com)
Preface
Combining two different scientific disciplines – morphology and immunochemistry – immunohistochemistry has developed as an important instrument in research and clinical pathology. A basic understanding of underlying principles and potential problems is unavoidable if you want to be successful in your use of immunohistochemistry, as well as in getting your papers published and your research grants funded. While many excellent texts and monographs exist which cover various aspects of immunohistochemistry, the lack of a concise comprehensive guide to using these methods was a major motivation for writing this book. Our intention was to create an easy-to-read and focused resource based on state-of-the-art information for a broad audience ranging from students and technical assistants to experienced researchers. This handbook has a concise format, with protocols and instructions for methods immediately following the short introductory theoretical material in each chapter. Being conscious of the growing role of Internet as an information source, we have found
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