Signal Transduction Immunohistochemistry Methods and Protocols
This second edition volume provides detailed protocols that address the challenges of signal-transduction IHC. This book delves into chapters that discuss the nature of signal transduction phenomena and approaches to making phosphor-specific antibodies, a
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Alexander E. Kalyuzhny Editor
Signal Transduction Immunohistochemistry Methods and Protocols Second Edition
Methods
in
Molecular Biology
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Signal Transduction Immunohistochemistry Methods and Protocols Second Edition
Edited by
Alexander E. Kalyuzhny Immunocytochemistry & Elispot Assays, Bio-Techne, Inc., Minneapolis, MN, USA
Editor Alexander E. Kalyuzhny Immunocytochemistry & Elispot Assays Bio-Techne, Inc. Minneapolis, MN, USA
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-6757-5 ISBN 978-1-4939-6759-9 (eBook) DOI 10.1007/978-1-4939-6759-9 Library of Congress Control Number: 2016962312 © Springer Science+Business Media LLC 2011, 2017 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Printed on acid-free paper This Humana Press imprint is published by Springer Nature The registered company is Springer Science+Business Media LLC The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A
Preface This is the second edition of the Signal Transduction Immunohistochemistry that we decided to put together after the success of the first edition, which received extremely positive feedback from researchers worldwide. During the years following the publication of the first edition, immunohistochemistry (IHC) has not only remained one of the most valuable research and diagnostic tools in biomedical field; it has gained additional popularity among researchers from different disciplines. Despite the availability of novel detecting reagents and sophisticated imaging techniques, the detection of transiently expressed tissue targets remains a challenging task not only to novices but also to seasoned and experienced researchers. Catching the appearance of the elusive, short-lived molecules involved in signal transduction require
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