Signal Transduction Immunohistochemistry Methods and Protocols
Unlike detecting constitutively expressed targets, immunohistochemical detection of labile, low abundance, and short-lived signal transduction molecules can be a very difficult task. In Signal Transduction Immunohistochemistry: Methods and Protocols
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Molecular Biology™
Series Editor John M. Walker School of Life Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
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Signal Transduction Immunohistochemistry Methods and Protocols Edited by
Alexander E. Kalyuzhny R&D Systems, Inc., Minneapolis, MN, USA
Editor Alexander E. Kalyuzhny, Ph.D. R & D Systems, Inc. Minneapolis, MN USA [email protected]
ISSN 1064-3745 e-ISSN 1940-6029 ISBN 978-1-61779-023-2 e-ISBN 978-1-61779-024-9 DOI 10.1007/978-1-61779-024-9 Springer New York Dordrecht Heidelberg London Library of Congress Control Number: 2011921260 © Springer Science+Business Media, LLC 2011 All rights reserved. This work may not be translated or copied in whole or in part without the written permission of the publisher (Humana Press, c/o Springer Science+Business Media, LLC, 233 Spring Street, New York, NY 10013, USA), except for brief excerpts in connection with reviews or scholarly analysis. Use in connection with any form of information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed is forbidden. The use in this publication of trade names, trademarks, service marks, and similar terms, even if they are not identified as such, is not to be taken as an expression of opinion as to whether or not they are subject to proprietary rights. While the advice and information in this book are believed to be true and accurate at the date of going to press, neither the authors nor the editors nor the publisher can accept any legal responsibility for any errors or omissions that may be made. The publisher makes no warranty, express or implied, with respect to the material contained herein. Printed on acid-free paper Humana Press is part of Springer Science+Business Media (www.springer.com)
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Preface Immunohistochemistry (IHC) is one the most valuable research and diagnostic tools in biomedical research. Unlike detecting constitutively expressed targets, immunohistochemical detection of labile, low abundance, and short-lived signal transduction molecules appears to be a very challenging task. This book represents a set of detailed protocols written by IHC experts addressing the challenges of signal transduction immunohistochemistry (ST-IHC); because it would be fair to say that ST-IHC as a discipline is in its infancy and the chapters in the first part are of a more introductory nature which should help new investigators in their orientation in the field. The second part is dedicated to techniques used for the preservation of antigens and their unmasking. The third part presents protocols in digital imaging and image analysis of stained cells and tissues and high-throughput data collection and data analysis. The fourth part is focused on ST-IHC techniques used in neuroscience as well as cancer and stem cell research. And
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