In Vivo Fluorescence Imaging Methods and Protocols
This detailed volume includes a rich variety of applications using various instrumentations, probes, disease models, and targets in order to account for the multidisciplinary nature of the use of in vivo fluorescence imagine. The book also includes chapte
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Mingfeng Bai Editor
In Vivo Fluorescence Imaging Methods and Protocols
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
In Vivo Fluorescence Imaging Methods and Protocols
Edited by
Mingfeng Bai University of Pittsburgh, Pittsburgh, PA, USA
Editor Mingfeng Bai University of Pittsburgh Pittsburgh, PA, USA
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-3719-6 ISBN 978-1-4939-3721-9 (eBook) DOI 10.1007/978-1-4939-3721-9 Library of Congress Control Number: 2016939974 © Springer Science+Business Media New York 2016 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Printed on acid-free paper This Humana Press imprint is published by Springer Nature The registered company is Springer Science+Business Media LLC New York
Preface Fluorescence imaging is widely used for biomedical research due to its high sensitivity and resolution, as well as low instrument cost. Although conventionally used to study biology at cellular and tissue levels, fluorescence imaging is emerging as a valuable tool for investigating physiological and pathophysiological processes in living systems. In the clinical setting, fluorescence imaging techniques, particularly those utilizing light in the near infrared (NIR) spectroscopic region where deep tissue penetration and low interference by tissue autofluorescence are offered, have been successfully used to provide real-time guidance in oncologic surgery. Undoubtedly, in vivo fluorescence imaging will continue to significantly impact biological research, drug discovery, and clinical practice. The fast-growing field of in vivo fluorescence imaging has attracted new researchers, who often express the desire and need for detailed procedures, methods, and troubleshooting guides. This book was prepared to meet this need, which includes a rich variet
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