Live Cell Imaging Methods and Protocols
Now a routine tool in biomedical and life science research, live cell imaging has made major progress enabling this core biochemical, cell, and molecular biology technique to become even more powerful, versatile, and affordable. In Live Cell Imaging
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MO L E C U L A R BI O L O G Y
Series Editor John M. Walker School of Life Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For other titles published in this series, go to www.springer.com/series/7651
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Live Cell Imaging Methods and Protocols
Edited by
Dmitri B. Papkovsky University College Cork, Cork, Ireland
Editor Dmitri B. Papkovsky Department of Biochemistry University College Cork Cavanagh Pharmacy Bldg. College Road Cork Ireland [email protected]
ISSN 1064-3745 e-ISSN 1940-6029 ISBN 978-1-60761-403-6 e-ISBN 978-1-60761-404-3 DOI 10.1007/978-1-60761-404-3 Library of Congress Control Number: 2009939132 © Humana Press, a part of Springer Science+Business Media, LLC 2010 All rights reserved. This work may not be translated or copied in whole or in part without the written permission of the publisher (Humana Press, c/o Springer Science+Business Media, LLC, 233 Spring Street, New York, NY 10013, USA), except for brief excerpts in connection with reviews or scholarly analysis. Use in connection with any form of information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed is forbidden. The use in this publication of trade names, trademarks, service marks, and similar terms, even if they are not identified as such, is not to be taken as an expression of opinion as to whether or not they are subject to proprietary rights. While the advice and information in this book are believed to be true and accurate at the date of going to press, neither the authors nor the editors nor the publisher can accept any legal responsibility for any errors or omissions that may be made. The publisher makes no warranty, express or implied, with respect to the material contained herein. Printed on acid-free paper springer.com
Preface Live cell imaging has now become a routine tool in biomedical and life science research. It is hard to imagine an active academic research department, pharmaceutical or biotechnology company without access to this technology and without using it on a regular basis. Over the last decade, major progress in this area has been achieved, making this core biochemical, cell and molecular biology techniques even more versatile, affordable, and mature. On the other hand, we continue witnessing numerous new, breakthrough developments which advance this technology even further, extending its capabilities and measurement standards. A variety of advanced-imaging methodologies, probe chemistries, experimental procedures, dedicated instruments, integrated systems, and a large number of new applications have come to the fore very recently. One can mention, for example, ultra-high resolution methods breaking the canonical diffraction limits, multi-photon excitation imaging and sample manipulation (e.g., (un)caging, permeabilization), new chemically and genetically engineered probes for key markers and parameters of cellular function, multi-color imaging, specialized detection formats, custom-buil
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