Insights into the microbiota of larval and postlarval Pacific white shrimp ( Penaeus vannamei ) along early developmenta
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ORIGINAL ARTICLE
Insights into the microbiota of larval and postlarval Pacific white shrimp (Penaeus vannamei) along early developmental stages: a case in pond level Hailiang Wang1 · Jie Huang1 · Ping Wang2 · Ting Li2 Received: 1 April 2020 / Accepted: 31 July 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Increasing studies have revealed strong links among gut microbiota, health status, and shrimp development, but they mainly focus on the microbiota of Pacific white shrimp, Penaeus vannamei, during life stages from juveniles to adults. Little is known about shrimp microbiota dynamics at early developmental stages. In this study, with an aim to profile shrimp microbiota and its dynamics at stages nauplius, zoea, mysis, and early postlarva, we conducted a survey for the successful breeding processes in a commercial hatchery in China, sampled 33 samples including larval/postlarval shrimp, suspended substance in rearing water (SSRW), and nutrition supplements (i.e., algae and brine shrimp larvae) at stages N5, Z2, M2, and P2. The associated bacterial communities were sequenced and comparatively analyzed using high-throughput sequencing of bacterial 16S rRNA genes. Our case study results showed that bacterial community structures and compositions were strikingly different at stages N5, Z2, and P2, indicating the shift of microbiota at the three stages. Many taxa within Gamma-, Alphaproteobacteria, and Flavobacteriia classes were observed to be stage-specifically abundant and identified as taxonomic biomarkers potentially used to differentiate among shrimp at different early developmental stages. Summing up, these results shed light on larval/ postlarval microbiota and its dynamics at different early developmental stages, highlighting the potential roles of shrimp development in microbiota formation and shifting. Keywords Shrimp · Life stages · Microbiome · Taxonomic biomarkers
Introduction
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00438-020-01717-2) contains supplementary material, which is available to authorized users. * Jie Huang [email protected] 1
Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Key Laboratory of Maricultural Organism Disease Control, Ministry of Agriculture and Rural Affairs, Qingdao Key Laboratory of Mariculture Epidemiology and Biosecurity, Qingdao, China
Hainan Zhongzheng Aquatic Science and Technology Co. Ltd, Dongfang, China
2
Environmental microorganisms are important components with respect to productivity, nutrient cycling, and water quality in an aquaculture ecosystem (Moriarty 1997). As aquatic species, shrimp are constantly exposed to a variety of microorganisms in the ambient water, of which some are probiotic, and some are pathogenic. The intestine tract of shrimp also harbors a diverse bacterial community that could pla
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