Isolation and effect of Trichoderma citrinoviride Snef1910 for the biological control of root-knot nematode, Meloidogyne
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RESEARCH ARTICLE
Open Access
Isolation and effect of Trichoderma citrinoviride Snef1910 for the biological control of root-knot nematode, Meloidogyne incognita Haiyan Fan1, Meiling Yao1, Haiming Wang1, Di Zhao2, Xiaofeng Zhu1, Yuanyuan Wang3, Xiaoyu Liu4, Yuxi Duan1 and Lijie Chen1*
Abstract Background: Root-knot nematode is one of the most significant diseases of vegetable crops in the world. Biological control with microbial antagonists has been emerged as a promising and eco-friendly treatment to control pathogens. The aim of this study was to screen and identify novel biocontrol agents against root-knot nematode, Meloidogyne incognita. Results: A total of 890 fungal isolates were obtained from rhizosphere soil of different crops and screened by nematicidal activity assays. Snef1910 strain showed high virulence against second stage juveniles (J2s) of M. incognita and identified as Trichoderma citrinoviride by morphology analysis and biomolecular assay. Furthermore, T. citrinoviride Snef1910 significantly inhibited egg hatching with the hatching inhibition percentages of 90.27, 77.50, and 67.06% at 48, 72, and 96 h after the treatment, respectively. The results of pot experiment showed that the metabolites of T. citrinoviride Snef1910 significantly decreased the number of root galls, J2s, and nematode egg masses and J2s population density in soil and significantly promoted the growth of tomato plants. In the field experiment, the biocontrol application showed that the control efficacy of T. citrinoviride Snef1910 against root-knot nematode was more than 50%. Meanwhile, T. citrinoviride Snef1910 increased the tomato plant biomass. Conclusions: T. citrinoviride strain Snef1910 could be used as a potential biological control agent against root-knot nematode, M. incognita. Keywords: Fungal bioagent, Nematode disease management, Plant growth promotion, Tomato
Background Root-knot nematode (RKN) is one of the most seriously damaging plant-parasitic nematode in the world threatening to the growth and production of more than 5500 plants, including vegetable crops and weeds [1–3]. Among them, Meloidogyne incognita, M. arenaria, M. javanica, * Correspondence: [email protected] 1 Nematology Institute of Northern China, College of Plant Protection, Shenyang Agricultural University, Shenyang, China Full list of author information is available at the end of the article
and M. hapla are the four main root-knot nematodes that have been reported. M. incognita is the most serious with 51% of the affected crops and the highest distribution proportion among these four species [4]. With rapidly increased developing protected agriculture, high-value crops, such as tomato (Solanum lycopersicum cultivar L402 susceptible to M. incognita), are severely damaged by M. incognita and has led to severe losses in China [5]. Currently, chemical and physical measures have limited use for RKN disease management. Furthermore, as the
© The Author(s). 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 Inter
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