Laser-assisted biofabrication in tissue engineering and regenerative medicine
- PDF / 911,749 Bytes
- 15 Pages / 584.957 x 782.986 pts Page_size
- 22 Downloads / 287 Views
Samantha M. Santoni Department of Biomedical & Chemical Engineering, Syracuse University, New York 13244, USA
Bruce Z. Gao Department of Bioengineering, Clemson University, South Carolina 29634, USA
Costas P. Grigoropoulos Department of Mechanical Engineering, University of California, Berkeley, California 94720, USA
Zhen Maa) Department of Biomedical & Chemical Engineering and Syracuse Biomaterials Institute, Syracuse University, New York 13244, USA (Received 24 August 2016; accepted 15 November 2016)
Controlling the spatial arrangement of biomaterials and living cells provides the foundation for fabricating complex biological systems. Such level of spatial resolution (less than 10 lm) is difficult to be obtained through conventional cell processing techniques, which lack the precision, reproducibility, automation, and speed required for the rapid fabrication of engineered tissue constructs. Recently, laserassisted biofabrication techniques are being intensively developed with the use of computer-aided processes for patterning and assembling both living and nonliving materials with prescribed 2D or 3D organization. In this review, we discuss laser-assisted fabrication methods, including laser tweezers, multi-photon polymerization, laser-induced forward transfer (LIFT), matrix assisted pulsed laser evaporation (MAPLE), and laser ablation as well as their applications in biological science and biomedical engineering. These advanced technologies enable the precise manipulation of in vitro cellular microenvironments and the ability to engineer functional tissue constructs with high complexity and heterogeneity, which serve in regenerative medicine, pharmacology, and basic cell biology studies. Zhen Ma is currently an Assistant Professor in Department of Biomedical and Chemical Engineering in Syracuse University, entitled as Samuel and Carol Nappi Research Scholar. He received his Ph.D. in Bioengineering from Clemson University in 2011 and pursued postdoctoral studies in University of California, Berkeley. His past research focused on laser-based fabrication technologies and their applications in multicellular patterning and micro-tissue construction. By applying laser-assisted biofabrication to cardiovascular research, he was awarded as Siebel Postdoctoral Scholar and American Heart Association (AHA) Postdoctoral Fellow. His primary research interests are organ-on-chip systems, stem cell engineering and laser-assisted biofabrication.
Zhen Ma
I. INTRODUCTION
Tissue engineering unites both engineering and life science principles to advance the development of Contributing Editor: Gary L. Messing a) Address all correspondence to this author. e-mail: [email protected] DOI: 10.1557/jmr.2016.452
biological substitutes that can restore or improve the function of tissue and organs.1 The final goal is to create artificial three-dimensional (3D) scaffolds that sufficiently mimic the natural biological environments, thereby biological cells in the artificial environments can function as well as they would in the real tissue. For
Data Loading...
