Laser Capture Microdissection Methods and Protocols
This fully updated edition provides practical advice on how to carry out tissue-based laser microdissection successfully by using the different laser microdissection systems that are available and by applying a wide range of molecular technologies. The co
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Graeme I. Murray Editor
Laser Capture Microdissection Methods and Protocols Third Edition
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Laser Capture Microdissection Methods and Protocols Third Edition Edited by
Graeme I. Murray School of Medicine, Medical Sciences and Nutrition, University of Aberdeen, Aberdeen, UK
Editor Graeme I. Murray School of Medicine, Medical Sciences and Nutrition University of Aberdeen Aberdeen, UK
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-7557-0 ISBN 978-1-4939-7558-7 (eBook) https://doi.org/10.1007/978-1-4939-7558-7 Library of Congress Control Number: 2017963745 © Springer Science+Business Media, LLC 2018 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Printed on acid-free paper This Humana Press imprint is published by Springer Nature The registered company is Springer ScienceþBusiness Media, LLC The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.
Preface Laser microdissection techniques have revolutionized the ability of researchers in general and pathologists in particular to carry out molecular analysis on specific types of normal and diseased cells and to fully utilize the power of current molecular technologies including PCR, microarrays, next generation sequencing, and proteomics. The primary purpose of the third edition of this volume of Methods in Molecular Biology is to provide the reader with practical advice on how to carry out tissue-based laser microdissection successfully in their own laboratory using the different laser microdissection systems that are available and apply a wide range of molecular technologies. The individual chapters encompass detailed descri
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