Laser Capture Microdissection Methods and Protocols
Laser microdissection techniques have revolutionized the ability of researchers in general, and pathologists in particular, to carry out molecular analysis on specific types of normal and diseased cells and to fully utilize the power of current molecular
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Molecular Biology™
Series Editor John M. Walker School of Life Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
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Laser Capture Microdissection Methods and Protocols Second Edition
Edited by
Graeme I. Murray Department of Pathology, University of Aberdeen, Aberdeen, UK
Editor Graeme I. Murray, MB ChB, PhD, DSc, FRCPath Department of Pathology University of Aberdeen Aberdeen, UK [email protected]
ISSN 1064-3745 e-ISSN 1940-6029 ISBN 978-1-61779-162-8 e-ISBN 978-1-61779-163-5 DOI 10.1007/978-1-61779-163-5 Springer New York Heidelberg London Dordrecht Library of Congress Control Number: 2011931522 © Springer Science+Business Media, LLC 2011 All rights reserved. This work may not be translated or copied in whole or in part without the written permission of the publisher (Humana Press, c/o Springer Science+Business Media, LLC, 233 Spring Street, New York, NY 10013, USA), except for brief excerpts in connection with reviews or scholarly analysis. Use in connection with any form of information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed is forbidden. The use in this publication of trade names, trademarks, service marks, and similar terms, even if they are not identified as such, is not to be taken as an expression of opinion as to whether or not they are subject to proprietary rights. Printed on acid-free paper Humana Press is part of Springer Science+Business Media (www.springer.com)
Preface Laser microdissection techniques have revolutionized the ability of researchers in general, and pathologists in particular, to carry out molecular analysis on specific types of normal and diseased cells and to fully utilize the power of current molecular technologies, including PCR, microarrays, and proteomics. The primary purpose of the second edition of this volume of Methods in Molecular Biology is to provide the reader with practical advice on how to carry out tissue-based laser microdissection successfully in their own laboratory using the different laser microdissection systems that are available and to apply a wide range of molecular technologies. The individual chapters encompass detailed descriptions of the individual laser-based microdissection systems. The downstream applications of the laser microdissected tissue described in the book include PCR in its many different forms as well as gene expression analysis, including the application to microarrays and proteomics. The editor is especially grateful to all the contributing authors for the time and effort they have put into the individual chapters. The series editor John Walker has provided expert guidance through the editorial process while colleagues at Springer have been very helpful in dealing with all the publication related issues. Aberdeen, UK
Graeme I. Murray
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Contents Preface . . . . . . . . . . . . . . . . . . . . . . . . .
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