Mammary Stem Cells Methods and Protocols
The identification of normal and breast cancer stem cells has offered a new vision of this heterogeneous disease and new hopes for its prognosis and treatment. This volume provides an overview of recent developments in mammary stem cell research and discu
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Maria del Mar Vivanco Editor
Mammary Stem Cells Methods and Protocols
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Mammary Stem Cells Methods and Protocols Edited by
Maria del Mar Vivanco CIC bioGUNE, Technological Park of Bizkaia, Derio, Spain
Editor Maria del Mar Vivanco CIC bioGUNE Technological Park of Bizkaia Derio, Spain
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-2518-6 ISBN 978-1-4939-2519-3 (eBook) DOI 10.1007/978-1-4939-2519-3 Library of Congress Control Number: 2015939081 Springer New York Heidelberg Dordrecht London © Springer Science+Business Media New York 2015 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Printed on acid-free paper Humana Press is a brand of Springer Springer Science+Business Media LLC New York is part of Springer Science+Business Media (www.springer.com)
Preface The concept of stem cells was first postulated more than a century ago, and over the last few years it has become a focus of interest and discussion even in nonscientific circles. The fact that this book is dedicated to Mammary Stem Cells reflects the attention that these cells have recently received and, as a consequence, many of the variety of approaches that researchers are using to investigate their properties and function are discussed here. Studying stem cells from the healthy mammary gland may not appear as relevant as the intriguing work presently being carried out with adult stem cells from other tissues, such as the heart or the nervous system, which epitomizes the great hope and potential for regenerative medicine in the future. However, understanding the role that stem cells play during development and in the physiology of the mammary gland is not only interesting in its own right but also essential to understand the molecular mechanisms underl
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