Mining Myb transcription factors related to wood development in Larix olgensis
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ORIGINAL PAPER
Mining Myb transcription factors related to wood development in Larix olgensis Jiali Zhao1 · Huanhuan Xiong1 · Junhui Wang2 · Hanguo Zhang1 · Lei Zhang1
Received: 28 March 2019 / Accepted: 24 June 2019 © Northeast Forestry University 2019
Abstract Larix olgensis A.Henry is a fast-growing tree used for afforestation in northeastern China and has great ecological and economic value. For studying developmental genes in the xylem of this species, we investigated the Myb transcription factor family, one of the largest families of transcription factors in plants, which plays an important role in the regulation of lignification in plant secondary walls. By sequencing a L. olgensis cDNA library using the Illumina HiSeq2500 high-throughput sequencing platform, we obtained 58,683 unigene sequences, of which 16,554 unigenes were longer than 1000 bp, accounting for 28.2% of the total database. The alignment of these genes with the GO, COG, KEGG, Swiss-Prot and NR databases resulted in annotated 29,350 unigenes. We obtained a total of 1460 differentially expressed genes, of which 453 were upregulated Project funding: The work was supported by National Natural Science Foundation of China (31700595), the Fundamental Research Funds for the Central Universities (2572019BA13), the National High Technology Research and Development Program of China (2013AA102704) and the Fundamental Research Funds for the Central Universities (2572018AA01). The online version is available at https://www.springerlink.com Corresponding editor: Tao Xu. * Hanguo Zhang [email protected] * Lei Zhang [email protected] 1
State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, People’s Republic of China
2
State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing 100081, People’s Republic of China
and 1007 were downregulated at the two developmental stages analyzed. The gene annotations showed a wide range of biological functions and metabolic pathways. The 10 Myb transcription factors that were obtained from the differentially expressed genes were analyzed by real-time quantitative PCR (qRT-PCR). The results showed that four Myb transcription factors may be associated with xylem development in L. olgensis. Due to the large genome size of conifers, genomics research on these species has lagged behind that for other plant groups. Our data provide the basis for further studies on xylem development in L. olgensis. Keywords Larix olgensis · RNA-seq · Myb transcription factors · Differentially expressed genes
Introduction Larix olgensis A.Henry is a fast-growing tree species used for afforestation in northeastern China forest areas. Its economically valuable traits are thus an important focus for research and improvement. The use of conventional genetic engineering techniques with L. olgensis could not overcome the problem of the long breeding cycle for conventional tree breeding, and many important economic traits could only be fully and effectively as
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