Molecularly Imprinted Solid-Phase Extraction Coupled with High-Performance Liquid Chromatography for the Determination o
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Molecularly Imprinted Solid-Phase Extraction Coupled with High-Performance Liquid Chromatography for the Determination of Trace Trichlorfon and Monocrotophos Residues in Fruits Deqing Li 1 & Xin Zhang 1 & Feifan Kong 2 & Xuguang Qiao 1 & Zhixiang Xu 1
Received: 30 July 2016 / Accepted: 3 October 2016 # Springer Science+Business Media New York 2016
Abstract In this study, a novel molecularly imprinted polymer (MIL@MIP) that could selectively recognize the trichlorfon and monocrotophos was synthesized using metal-organic framework MIL-101 as support material. The prepared MIL@MIP was characterized by Fourier transform infrared, thermogravimetric analysis, scanning electron microscope, static adsorption, kinetic adsorption, and competitive adsorption analyses. Using this material as sorbent, a new method of molecularly imprinted solidphase extraction coupled with high performance liquid chromatography for simultaneous determination of trichlorfon and monocrotophos was established. Under optimal conditions, the limit of detection (LOD) of this method for trichlorfon and monocrotophos detection was 0.011 and 0.015 mg/kg, respectively. The relative standard deviation (RSD) for five replicates of 0.05 mg/L trichlorfon and monocrotophos mixed solutions was within the range of 1.8 to 3.7 %. Apple and pear samples spiked with the two kinds of organophosphate pesticides were extracted and determined by using this method with good recoveries ranging from 86.5 to 91.7 %. Moreover, this method was successfully applied for the detection of the two organophosphate pesticides in strawberry samples.
Xin Zhang and Deqing Li contributed equally to this work. * Xuguang Qiao [email protected] * Zhixiang Xu [email protected] 1
College of Food Science and Engineering, Shandong Agricultural University, Taian 271018, China
2
Shandong Wuzhou Detection Co., Ltd., Sishui 273200, China
Keywords Organophosphate pesticide . Metal-organic frameworks . Molecular imprinting technology . Solid-phase extraction . High-performance liquid chromatography
Introduction Organophosphate pesticides are widely used in agricultural production. However, their extensive use also gives rise to pesticide residues in plants, which has lately become a serious health problem because of their toxicity and potential carcinogenicity (Casida and Quistad 2004; Mulchandani et al. 2001). Therefore, it is crucial to develop an accurate and reliable detection method to prevent their negative effects on human health. In the past decades, several different technologies have been reported for the determination of organophosphorus pesticides such as gas chromatography (GC) with flame photometric detection (Cai et al. 1995; Liu et al. 2014) or nitrogen– phosphorus detection (Tian et al. 2014); gas chromatography coupled with tandem mass spectrometry (GC-MS) (Chen et al. 2009); high-performance liquid chromatography (HPLC) with diode array detection (Xue et al. 2015; Seebunrueng et al. 2014; Pirsaheb et al. 2013); liquid chromatography coupled with tandem mass spectrometr
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