Multifunctional hydrolysates from kenaf ( Hibiscus cannabinus L.) seed protein with high antihypertensive activity in vi
- PDF / 878,664 Bytes
- 12 Pages / 595.276 x 790.866 pts Page_size
- 3 Downloads / 160 Views
ORIGINAL PAPER
Multifunctional hydrolysates from kenaf (Hibiscus cannabinus L.) seed protein with high antihypertensive activity in vitro and in vivo Nurul Dhania Zaharuddin1 · Mohamad Ariff Hanafi1 · Chay Shyan Yea1 · Farah Salina Hussin1 · Shehu Muhammad Auwal2 · Mohammad Zarei3 · Shahrul Razid Sarbini4 · Wan Zunairah Wan Ibadullah1 · Roselina Karim5 · Nazamid Saari1 Received: 11 March 2020 / Accepted: 14 September 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract Kenaf (Hibiscus cannabinus L.) seed is an underutilized protein-rich resource considered as a by-product of the kenaf fiber processing industry. Its high protein content (34%) makes it a promising candidate as a source of bioactive protein hydrolysates. In this study, the potential of enzymatically hydrolyzed kenaf seed protein to generate multifunctional bioactive peptides was evaluated. Kenaf seed protein concentrate was hydrolyzed using four different proteolytic enzymes (papain, alcalase, bromelain, and flavourzyme) at their respective optimum pH and temperature. The choice of enzyme affected the bioactivities to a certain degree as KSPH were shown to possess high ACE inhibitory activity and low-to-moderate DPP-IV and antioxidant activity. Papain KSPH showed the highest ACE inhibitory activity with 95% inhibition compared to other enzymatic hydrolysates, and therefore was chosen for further investigation of its antihypertensive activity. Papain KSPH was profiled for its hydrophobicity by RP-HPLC and revealed that the majority of late-eluting fractions exerted the highest ACE inhibitory activity. Spontaneously hypertensive rats showed a decrease of approximately 18–46 mmHg in their systolic blood pressure (BP) from 0 to 24 h after oral administration of papain KSPH at dosages of 100 mg/kg, 300 mg/kg, and 500 mg/ kg. However, the effect was not dose-dependent. As a novel protein source, future research should aim to demonstrate the safety of kenaf seed protein and its hydrolysates, and validate its bioactivity through human intervention trials. Overall, kenaf seed protein has the potential to generate antihypertensive hydrolysates with multifunctional bioactivities as part of a functional food ingredient. Keywords Kenaf seed · Protein hydrolysate · Multifunctional bioactivity · Antihypertensive · Spontaneously hypertensive rat * Nazamid Saari [email protected] 1
Department of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia
2
Department of Biochemistry, Faculty of Basic Medical Sciences, Bayero University Kano, P.M.B 3011, Kano, Nigeria
3
Department of Food Science and Technology, School of Industrial Technology, Faculty of Applied Sciences, Universiti Teknologi MARA, 40450 Shah Alam, Selangor, Malaysia
4
Department of Crop Science, Faculty of Agricultural and Food Sciences, Universiti Putra Malaysia Bintulu Campus, Jalan Nyabau, 97008 Bintulu, Sarawak, Malaysia
5
Department of Food Technology, Faculty of Food Science and Technology,
Data Loading...
