Natural Antibodies Methods and Protocols
This volume looks at the role of natural antibodies in pathogen elimination, cell survival, inflammation, cancer, and autoimmunity. The chapters in this book cover numerous topics, such as isolation of natural antibodies; methods for separating natural an
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Srinivas V. Kaveri Jagadeesh Bayry Editors
Natural Antibodies Methods and Protocols
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Natural Antibodies Methods and Protocols
Edited by
Srinivas V. Kaveri Centre de Recherche des Cordeliers INSERM, PARIS Paris, France
Jagadeesh Bayry Centre de Recherche des Cordeliers INSERM, PARIS Paris, France
Editors Srinivas V. Kaveri Centre de Recherche des Cordeliers INSERM, PARIS Paris, France
Jagadeesh Bayry Centre de Recherche des Cordeliers INSERM, PARIS Paris, France
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-7179-4 ISBN 978-1-4939-7180-0 (eBook) DOI 10.1007/978-1-4939-7180-0 Library of Congress Control Number: 2017943968 © Springer Science+Business Media LLC 2017 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Printed on acid-free paper This Humana Press imprint is published by Springer Nature The registered company is Springer Science+Business Media LLC The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.
Preface Natural antibodies, belonging to isotypes IgM, IgG, and IgA, were discovered nearly half a century ago. Natural antibodies are those that are detected in the serum of healthy organisms in the absence of pathology or intentional immunization. Most natural antibodies bind to one or more autoantigens and are called as natural autoantibodies. The importance of natural autoantibodies in immune regulation and in therapeutic application is now well established. The role of natural antibodies in antigen presentation, pathogen elimination, cell survival and homeostasis, inflammation, cancer, and autoimmunity is exhaustively documented. While the basic properties
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