Synthetic Antibodies Methods and Protocols
This detailed volume presents a set of protocols useful for researchers in the field of recombinant immunoglobulin and alternative scaffold engineering, aptamer development, and generation of molecularly imprinted polymers (MIPs). Part I includes methods
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Thomas Tiller Editor
Synthetic Antibodies Methods and Protocols
Methods
in
Molecular Biology
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Synthetic Antibodies Methods and Protocols
Edited by
Thomas Tiller MorphoSys AG, Discovery Alliances & Technologies, Planegg, Germany
Editor Thomas Tiller MorphoSys AG, Discovery Alliances & Technologies Planegg, Germany
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-6855-8 ISBN 978-1-4939-6857-2 (eBook) DOI 10.1007/978-1-4939-6857-2 Library of Congress Control Number: 2017933077 © Springer Science+Business Media LLC 2017 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Printed on acid-free paper This Humana Press imprint is published by Springer Nature The registered company is Springer Science+Business Media LLC The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.
Dedication Dedicated to Isi, Josh & Family. ...and Rea. Thanks for inspirational, wise, and humorous support.
Preface Antibodies are important tools that are used extensively in basic biomedical research, in diagnostics, and in the treatment of diseases. Traditionally, the production of antibodies relies on the immunization of an animal. For example, for the generation of monoclonal antibodies by the hybridoma technology, usually mice and rats are preferred. For polyclonal antibody production, larger mammals (e.g., rabbits, sheep, and goats) are used as the relatively huge amount of serum that can be collected from these animals serves as a rich source for antibody purification. These antibodies are all based on an immunoglobulin scaffold and are derived from a genuine in vivo immune response. Despite their widespread applications
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