Neisseria gonorrhoeae Methods and Protocols
This detailed book addresses the continual development and application of new methods and protocols to understand the biology of the pathogen Neisseria gonorrhoeae. Beginning with gonococcus biology and antimicrobial resistance, the volume continues
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Myron Christodoulides Editor
Neisseria gonorrhoeae Methods and Protocols
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Neisseria gonorrhoeae Methods and Protocols
Edited by
Myron Christodoulides Molecular Microbiology Group, Academic Unit of Clinical and Experimental Sciences, Faculty of Medicine, University of Southampton, Southampton, UK
Editor Myron Christodoulides Molecular Microbiology Group Academic Unit of Clinical and Experimental Sciences Faculty of Medicine University of Southampton Southampton, UK
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-9495-3 ISBN 978-1-4939-9496-0 (eBook) https://doi.org/10.1007/978-1-4939-9496-0 © Springer Science+Business Media, LLC, part of Springer Nature 2019 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors, and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Cover Caption: Neisseria gonorrhoeae infection remodels microvilli in human 3-dimensional (3-D) endometrial cell model. Scanning electron microscopy image of 3-D human cells infected with N. gonorrhoeae MS11 at a multiplicity of infection of 10 for 4h. Gonococci were pseudo-colored red using Photoshop C5.1 software (Adobe). Image courtesy of Paweł Łaniewski and Melissa M. Herbst-Kralovetz. This Humana Press imprint is published by the registered company Springer Science+Business Media, LLC part of Springer Nature The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.
Preface Introduction “Gonorrhoea is as old as the world” (George Luys) [1]. Neisseria gonorrhoeae (gonococcus) is an obligate human pathogen that has coexisted with humanity and causes the sexually transmitted disease gonorrhea. Gonococci infect principally the mucosal epithelium of the genitourinary tra
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