Novel Graphene Biosensor Based on the Functionalization of Multifunctional Nano-bovine Serum Albumin for the Highly Sens
- PDF / 1,048,153 Bytes
- 13 Pages / 595.276 x 790.866 pts Page_size
- 84 Downloads / 182 Views
ARTICLE
Cite as Nano-Micro Lett. (2019) 11:20 Received: 29 December 2018 Accepted: 20 February 2019 © The Author(s) 2019
https://doi.org/10.1007/s40820-019-0250-8
Novel Graphene Biosensor Based on the Functionalization of Multifunctional Nano‑bovine Serum Albumin for the Highly Sensitive Detection of Cancer Biomarkers Lin Zhou1, Kun Wang1, Hao Sun1, Simin Zhao2, Xianfeng Chen3, Dahong Qian2, Hongju Mao1 *, Jianlong Zhao1 * Lin Zhou and Kun Wang have contributed equally contributed to this work. * Hongju Mao, [email protected]; Jianlong Zhao, [email protected] State Key Laboratory of Transducer Technology; Key Laboratory of Terahertz Solid‑State Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050, People’s Republic of China
1
2
3
School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200240, People’s Republic of China School of Electronic Engineering, Bangor University, Bangor LL57 1UT, UK
HIGHLIGHTS • A simple and convenient graphene bio-interface was designed by using multifunctional nano-denatured bovine serum albumin (nanodBSA) film. • Highly sensitive cancer biomarker detection in diluted serum at the femtogram per milliliter level was achieved using the nano-dBSA functionalized graphene field-effect transistor.
ABSTRACT A simple, convenient, and highly sensitive bio-interface for graphene
field-effect transistors (GFETs) based on multifunctional nano-denatured bovine serum albumin (nano-dBSA) functionalization was developed to target cancer bio‑ markers. The novel graphene–protein bioelectronic interface was constructed by heating to denature native BSA on the graphene substrate surface. The formed nanodBSA film served as the cross-linker to immobilize monoclonal antibody against car‑ cinoembryonic antigen (anti-CEA mAb) on the graphene channel activated by EDC and Sulfo-NHS. The nano-dBSA film worked as a self-protecting layer of graphene to prevent surface contamination by lithographic processing. The improved GFET
biosensor exhibited good specificity and high sensitivity toward the target at an ultralow concentration of 337.58 fg mL−1. The electrical detection of the binding of CEA followed the Hill model for ligand–receptor interaction, indicating the negative binding cooperativity between CEA and anti-CEA mAb with a dissociation constant of 6.82 × 10−10 M. The multifunctional nano-dBSA functionalization can
confer a new function to graphene-like 2D nanomaterials and provide a promising bio-functionalization method for clinical application in biosensing, nanomedicine, and drug delivery. KEYWORDS Bio-interface; Multifunctional denatured BSA; GFET biosensor; Cancer biomarker
Vol.:(0123456789)
13
20
Page 2 of 13
1 Introduction Cancer is a major public health problem worldwide. For many cancers, it can take 20–30 years for initial lesions to progress to late-stage disease [1]. Early detection is the key to cancer control, especially in reducing incidence rates and cancer-related deaths [2].
Data Loading...
