Overexpression of CcNAC1 gene promotes early flowering and enhances drought tolerance of jute ( Corchorus capsularis L .
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ORIGINAL ARTICLE
Overexpression of CcNAC1 gene promotes early flowering and enhances drought tolerance of jute (Corchorus capsularis L.) Gaoyang Zhang 1,2 & Siqi Huang 1 & Chao Zhang 1 & Defang Li 1 & Yingbao Wu 2 & Jielou Deng 2 & Shilian Shan 2 & Jianmin Qi 3 Received: 19 June 2020 / Accepted: 12 October 2020 # Springer-Verlag GmbH Austria, part of Springer Nature 2020
Abstract Drought is the main factor that significantly affects plant growth and has devastating effects on crop production of jute. NAC (NAM, ATAF, and CUC2) transcription factors (TFs) are a large gene family in plants that have been shown to play many important roles in regulating developmental processes and abiotic stress resistance. In this study, a NAC transcription factor, CcNAC1, was cloned and characterized its function in jute. RT-qPCR analysis showed that CcNAC1 expression peaks after 8 h of drought stress. CcNAC1 overexpression and knockdown plants were created by Agrobacterium-mediated genetic transformation. PCR and southern hybridization results indicate that the CcNAC1 gene was integrated into the genome of jute. Overexpression of the CcNAC1 gene sped up the plant growth, promoted early flowering, and increased drought tolerance compared to the control plants. 3-Ketoacyl-CoA synthase (KCS) gene expression level increased significantly in the CcNAC1-overexpression plants and decreased in knockdown plants, which showed that CcNAC1 transcription factor regulated KCS gene expression. Yeast-2-Hybrid (Y2H) assays validated the physical interaction between CcNAC1 and KCS. The results provide relatively comprehensive information on the molecular mechanisms of CcNAC1 gene underlying the regulation of plant growth and drought stress resistance, and indicate that CcNAC1 acts as a positive regulator in drought tolerance in jute (Corchorus capsularis L.). Keywords Jute (Corchorus capsularis L.) . NAC transcription factor . Drought stress . Yeast-2-hybrid . Molecular mechanism
Introduction Jute (Corchorus capsularis L.) is a plant that is conventionally used for the production of natural fibers, paper, chemical fertilizers, building materials, and medicine. Fibers from jute
Handling Editor: Néstor Carrillo Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00709-020-01569-y) contains supplementary material, which is available to authorized users. * Defang Li [email protected] * Jianmin Qi [email protected] 1
Chinese Academy of Agricultural Sciences, Institute of Bast Fiber Crops, Changsha, China
2
College of Life Sciences, Shangrao Normal University, Shangrao, China
3
Institute of Genetics and Breeding, Fujian Agriculture and Forestry University, Fuzhou, China
have good glossiness, high drapability, good hygroscopicity, high water dispersibility, good biodegradability, and other beneficial characteristics. The advantages of jute over other plants have led to it being widely applied in various fields. Drought is among the most important abiotic factors that affect the growth and development of jut
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