Photosynthesis Research Protocols

The capture of sunlight by photosynthetic organisms supplies an enormous amount of the energy required to develop and sustain life on the planet, making photosynthesis one of the most important biological phenomenon on Earth. Updating the popular first ed

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Photosynthesis Research Protocols Second Edition

Edited by Robert Carpentier

Methods

in

Molecular Biology™

Series Editor John M. Walker School of Life Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK



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Photosynthesis Research Protocols Second Edition

Edited by

Robert Carpentier Département de Chimie Biologie, Université du Québec à Trois-Rivières, Trois-Rivières, Québec, Canada

Editor Dr. Robert Carpentier Département de Chimie Biologie Université du Québec à Trois-Rivières Trois-Rivières, Québec Canada [email protected]

ISSN 1064-3745 e-ISSN 1940-6029 ISBN 978-1-60761-924-6 e-ISBN 978-1-60761-925-3 DOI 10.1007/978-1-60761-925-3 Springer New York Dordrecht Heidelberg London Library of Congress Control Number: 2010938370 © Springer Science+Business Media, LLC 2011 All rights reserved. This work may not be translated or copied in whole or in part without the written permission of the publisher (Humana Press, c/o Springer Science+Business Media, LLC, 233 Spring Street, New York, NY 10013, USA), except for brief excerpts in connection with reviews or scholarly analysis. Use in connection with any form of information storage and retrieval, electronic adaptation, computer software, or by similar or ­dissimilar methodology now known or hereafter developed is forbidden. The use in this publication of trade names, trademarks, service marks, and similar terms, even if they are not identified as such, is not to be taken as an expression of opinion as to whether or not they are subject to proprietary rights. While the advice and information in this book are believed to be true and accurate at the date of going to press, ­neither the authors nor the editors nor the publisher can accept any legal responsibility for any errors or omissions that may be made. The publisher makes no warranty, express or implied, with respect to the material contained herein. Printed on acid-free paper Humana Press is part of Springer Science+Business Media (www.springer.com)

Preface Photosynthesis is one of the most important biological phenomena on Earth. The capture of sunlight by photosynthetic organisms supplies most of the energy required to develop and sustain life on the planet. Not only is photosynthesis at the heart of plant bioenergetics, it is also fundamental to plant productivity and biomass. Photosynthetic carbon fixation and oxygen evolution directly intervene into many environmental aspects, such as the global atmospheric CO2 level and global climate. Therefore, it is not surprising that a large effort is devoted to photosynthesis research. Photosynthesis, in itself, is of great interest to a multidisciplinary field of research involving agriculture, biochemistry, biotechnology, botany, cell biology, environmental sciences, forestry, plant genetics, plant molecular biology, photobiology, photophysics, photoprotection, plant physiology, plant stress, etc. This book is thus intended as a source of inf