Salamanders in Regeneration Research Methods and Protocols
This detailed volume focuses on best practices and conditions for maintaining the most commonly used salamander species in the laboratory. Salamanders in Regeneration Research: Methods and Protocols guides readers through experimental manipulations in viv
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Anoop Kumar András Simon Editors
Salamanders in Regeneration Research Methods and Protocols
METHODS
IN
M O L E C U L A R B I O LO G Y
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Salamanders in Regeneration Research Methods and Protocols
Edited by
Anoop Kumar Institute of Structural and Molecular Biology, Division of Biosciences, University College London, London, UK
András Simon Department of Cell and Molecular Biology, Karolinska Institute, Stockholm, Sweden
Editors Anoop Kumar Institute of Structural and Molecular Biology, Division of Biosciences University College London London, UK
András Simon Department of Cell and Molecular Biology Karolinska Institute Stockholm, Sweden
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-2494-3 ISBN 978-1-4939-2495-0 (eBook) DOI 10.1007/978-1-4939-2495-0 Library of Congress Control Number: 2015931955 Springer New York Heidelberg Dordrecht London © Springer Science+Business Media New York 2015 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Printed on acid-free paper Humana Press is a brand of Springer Springer Science+Business Media LLC New York is part of Springer Science+Business Media (www.springer.com)
Preface Many of the most fundamental discoveries in experimental biology, such as the embryonic organizers, neuronal specificity, nerve guidance, and units of DNA transcription, originate from salamander research. Salamanders are the only tetrapods capable of repeatedly regenerating entire limbs as adults, and they also display the widest range of regeneration capacities of other complex tissues and organs. These animals constitute unique models for understanding critical processes underlying morphological and functional restoration of lost or damaged structures in vertebrates. The present volume focuses on this particular aspect of salama
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