Kidney Research Experimental Protocols

This second edition expands upon the previous volume with additional emphasis on recent innovation in basic renal research and has a more holistic approach on associated disorders such as complications associated with peritoneal dialysis, ischemic acute k

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Tim D. Hewitson Edward R. Smith Stephen G. Holt Editors

Kidney Research Experimental Protocols Second Edition

METHODS

IN

MOLECULAR BIOLOGY

Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK

For further volumes: http://www.springer.com/series/7651

Kidney Research Experimental Protocols Second Edition

Edited by

Tim D. Hewitson Department of Nephrology, Royal Melbourne Hospital, Melbourne, VIC, Australia

Edward R. Smith Department of Nephrology, Royal Melbourne Hospital, Melbourne, VIC, Australia

Stephen G. Holt Department of Nephrology, Royal Melbourne Hospital, Melbourne, VIC, Australia

Editors Tim D. Hewitson Department of Nephrology Royal Melbourne Hospital Melbourne, VIC, Australia

Edward R. Smith Department of Nephrology Royal Melbourne Hospital Melbourne, VIC, Australia

Stephen G. Holt Department of Nephrology Royal Melbourne Hospital Melbourne, VIC, Australia

ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-3351-8 ISBN 978-1-4939-3353-2 DOI 10.1007/978-1-4939-3353-2

(eBook)

Library of Congress Control Number: 2015957191 Springer New York Heidelberg Dordrecht London © Springer Science+Business Media New York 2016 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Printed on acid-free paper Humana Press is a brand of Springer Springer Science+Business Media LLC New York is part of Springer Science+Business Media (www.springer.com)

Preface Acute and chronic forms of kidney disease are major causes of morbidity and mortality worldwide. In compiling this volume, we have attempted to collate both established and novel experimental methods that can be used to study this debilitating condition. The first edition of this book gathered together a collection of techniques that focused on solving the inherent problems that exist in renal research, most notably the complexity of renal anatomy and physiology and the associated plethora of different