Chimera Research Methods and Protocols
This volume addresses challenging new questions surrounding stem cell-based chimera research. This book is organized into three parts: Part One provides readers with a summary of different human donor cell types. The chapters in this section discuss ways
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Insoo Hyun Alejandro De Los Angeles Editors
Chimera Research Methods and Protocols
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Chimera Research Methods and Protocols
Edited by
Insoo Hyun Department of Bioethics, Case Western Reserve University, School of Medicine, Cleveland, OH, USA
Alejandro De Los Angeles Department of Psychiatry, Yale University School of Medicine, New Haven, CT, USA
Editors Insoo Hyun Department of Bioethics Case Western Reserve University School of Medicine Cleveland, OH, USA
Alejandro De Los Angeles Department of Psychiatry Yale University School of Medicine New Haven, CT, USA
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-9523-3 ISBN 978-1-4939-9524-0 (eBook) https://doi.org/10.1007/978-1-4939-9524-0 © Springer Science+Business Media, LLC, part of Springer Nature 2019 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors, and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Cover illustration: Chimera Period: Six Dynasties (220 - 589) Date: 5th century Culture: China Medium: Stone Classification: Sculpture Credit Line: Fletcher Fund, 1973 Accession Number: 63.224.1 Dimensions: H. 21 1/2 in (54.6 cm); W. 18 in (45.7 cm); L. 18 1/2 in (47 cm). This Humana imprint is published by the registered company Springer Science+Business Media, LLC, part of Springer Nature. The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.
Preface In the ancient imagination, the chimera was a monster comprised of parts from a lion, a goat, and a serpent. In modern biomedical research, chimeras are entities made up of cells from two or more zygotes of the same or different species. Experimental chimeras comprised of cells from two individuals, particularly in the mou
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