Plant Cytogenetics Methods and Protocols
This volume covers a range of methods used in plant cytogenetics, beginning with basic analysis of chromosomes and visualizing gene locations, to manipulating and dissecting chromosomes, and then focusing on less understood features of chromosomes such as
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Shahryar F. Kianian Penny M. A. Kianian Editors
Plant Cytogenetics Methods and Protocols
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Plant Cytogenetics Methods and Protocols
Edited by
Shahryar F. Kianian University of Minnesota, St. Paul, MN, USA
Penny M.A. Kianian University of Minnesota, St. Paul, MN, USA
Editors Shahryar F. Kianian University of Minnesota St. Paul, MN, USA
Penny M.A. Kianian University of Minnesota St. Paul, MN, USA
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-3620-5 ISBN 978-1-4939-3622-9 (eBook) DOI 10.1007/978-1-4939-3622-9 Library of Congress Control Number: 2016941227 © Springer Science+Business Media New York 2016 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Photo Credit: Dr. Adam J. Lukaszewski, Dr. D. Kopecky, and Dr. G. Linc. Printed on acid-free paper This Humana Press imprint is published by Springer Nature The registered company is Springer Science+Business Media LLC New York
Foreword: The Modern Cytogenetics Tool Box—A Picture Is Still Worth a Thousand Words Cytogenetics is a stepping stone toward a full understanding of genetics. The report of in situ hybridization by Pardue and Gall in 1969 introduced a new era combining cytogenetics and molecular biology. An important feature was that allelic variation was not required for placing important genes to chromosome—and their place could be directly visualized with the light microscope. The narrative of this book takes the reader from the neoclassical to modern technologies for visualizing chromosomes, chromosome segments, and DNA strands. Many of the techniques require modest equipment and other resources. The book addresses complex situations including polyploidy in species such as oat and wheat. The chapters in this book are clearly written and provide useful
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