Plant Embryo Culture Methods and Protocols
A great fascination for biologists, the study of embryo development provides indispensable information concerning the origins of the various forms and structures that make up an organism, and our ever-increasing knowledge gained through the study of plant
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Series Editor John M. Walker School of Life Sciences University of Hertfordshire Hartfield, Hertfordshire, AL10 9AB, UK
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Plant Embryo Culture Methods and Protocols Edited by
Trevor A. Thorpe and Edward C. Yeung Department of Biological Sciences, University of Calgary, Calgary, Alberta, Canada
Editors Trevor A. Thorpe Department of Biological Sciences University of Calgary Calgary, Alberta Canada [email protected]
Edward C. Yeung Department of Biological Sciences University of Calgary Calgary, Alberta Canada [email protected]
ISSN 1064-3745 e-ISSN 1940-6029 ISBN 978-1-61737-987-1 e-ISBN 978-1-61737-988-8 DOI 10.1007/978-1-61737-988-8 Springer New York London Dordrecht Heidelberg © Springer Science+Business Media, LLC 2011 All rights reserved. This work may not be translated or copied in whole or in part without the written permission of the publisher (Humana Press, c/o Springer Science+Business Media, LLC, 233 Spring Street, New York, NY 10013, USA), except for brief excerpts in connection with reviews or scholarly analysis. Use in connection with any form of information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed is forbidden. The use in this publication of trade names, trademarks, service marks, and similar terms, even if they are not identified as such, is not to be taken as an expression of opinion as to whether or not they are subject to proprietary rights. Printed on acid-free paper Humana Press is part of Springer Science+Business Media (www.springer.com)
Preface Embryo development is a great fascination for biologists, irrespective of their discipline. While technically challenging, the study of embryo development provides indispensable information concerning the origins of the various forms and structures that make up the organism. Moreover, many useful applications have been derived from the knowledge gained through the study of plant embryology. The introduction of in vitro culture of zygotic embryos has greatly facilitated the study of embryo development and has allowed for studies not possible in vivo. In vitro culture has in and of itself proven invaluable as a method in plant science for both applied and basic research. The main purpose of this book is to provide a ready source of information for culturing zygotic embryos for diffe rent types of studies, both theoretical and practical. Although some procedures described here are standard, we expect that the assembly under the same theme will provide a quick reference source for our readers. A range of related topics have been selected. Our intention is that the protocols in this volume will serve as reference materials that can be used to help others develop their own customized methods for different species and for different purposes. It is not intended to be exhaustive. The book chapters are divided into five main sections: (
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