Plant Metabolomics Methods and Protocols
This detailed book aims to address the need for standardizing key steps in plant metabolomics research that is still considered a critical issue in the field. The collection unites diverse mass spectrometry (MS)-based protocols, from sample preparat
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Carla António Editor
Plant Metabolomics Methods and Protocols
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Plant Metabolomics Methods and Protocols
Edited by
Carla António Plant Metabolomics Laboratory, Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa (ITQB NOVA), Oeiras, Portugal
Editor Carla Anto´nio Plant Metabolomics Laboratory Instituto de Tecnologia Quı´mica e Biolo´gica Anto´nio Xavier Universidade Nova de Lisboa (ITQB NOVA) Oeiras, Portugal
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-7818-2 ISBN 978-1-4939-7819-9 (eBook) https://doi.org/10.1007/978-1-4939-7819-9 Library of Congress Control Number: 2018940501 © Springer Science+Business Media, LLC, part of Springer Nature 2018 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Printed on acid-free paper This Humana Press imprint is published by the registered company Springer Science+Business Media, LLC part of Springer Nature. The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.
Dedication In Memory of the Late Professor Tsutomu Masujima. Carla Anto´nio
Preface Studies of the plant metabolome include the analysis of a wide range of chemical species with very diverse physico-chemical properties, and therefore, powerful analytical methodologies are required for the separation, characterization, and quantification of this vast compound diversity present in plant biological samples. Due to its high sensitivity, mass spectrometry (MS) is one main analytical technology driving plant metabolomics studies, typically coupled to liquid chromatography (LC-MS) and gas chromatography (GC-MS). However, currently, there is a
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