Plant Proteostasis Methods and Protocols
This volume is a collection of detailed protocols describing state-of-art approaches that will facilitate the understanding of protein homeostasis in plant stress responses and development. Plant Proteostasis: Methods and Protocols is broken int
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L. Maria Lois Rune Matthiesen Editors
Plant Proteostasis Methods and Protocols
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Plant Proteostasis Methods and Protocols
Edited by
L. Maria Lois Development Program, CRAG (CSIC-IRTA-UAB-UB) Edifici CRAG-Campus UAB, Bellaterra (Cerdanyola del Vallés), Barcelona, Spain
Rune Matthiesen Computational and Experimental Biology Group, National Health Institute Dr. Ricardo Jorge, IP, Lisbon, Portugal
Editors L. Maria Lois Development Program CRAG (CSIC-IRTA-UAB-UB) Edifici CRAGCampus UAB Bellaterra (Cerdanyola del Vallés) Barcelona, Spain
Rune Matthiesen Computational and Experimental Biology Group National Health Institute Dr. Ricardo Jorge IP, Lisbon, Portugal
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-3757-8 ISBN 978-1-4939-3759-2 (eBook) DOI 10.1007/978-1-4939-3759-2 Library of Congress Control Number: 2016943033 © Springer Science+Business Media New York 2016 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Printed on acid-free paper This Humana Press imprint is published by Springer Nature The registered company is Springer Science+Business Media LLC New York
Preface Plant responses to environmental stimuli and developmental transitions are regulated by complex regulatory networks that deliver the specific physiological outcome to assure plant survival. These networks include transcriptional regulation but also sophisticated posttranslational modifications that aim to regulate protein activity. In contrast to transcriptional regulation, which involves de novo protein synthesis, posttranslational modifications modulate protein activity in short time periods facilitating rapid cell responses. The molecular consequences of posttranslational modifications on the protein target are highly variable and include
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