Porous PtAg nanoshells/reduced graphene oxide based biosensors for low-potential detection of NADH
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ORIGINAL PAPER
Porous PtAg nanoshells/reduced graphene oxide based biosensors for low-potential detection of NADH Hongxiao Yang 1 & Jiagang Hou 2 & Zhaohui Wang 1 & Qiuxia Zhou 1 & Caixia Xu 1 Received: 3 January 2020 / Accepted: 22 August 2020 # Springer-Verlag GmbH Austria, part of Springer Nature 2020
Abstract A superior NADH sensing platform was constructed based on porous PtAg nanoshells supported on reduced graphene oxide (PtAg/rGO) in the absence of any enzymes and redox mediators. The PtAg/rGO composite was prepared via onestep reduction combined with galvanic replacement reaction. The as-made PtAg/rGO assembles multiple structural advantages of coherent conductive matrix, rich electroactive sites, and high specific surface area, accompanied by the unique alloying effect. The PtAg/rGO possesses adequate active reaction sites and fluent electron transport pathway towards the electrocatalytic NADH oxidation, thus presenting significantly increased oxidation current and negative shift of 330 mV in applied potential relative to the bare GCE. By virtues of the outstanding electrocatalytic activity, PtAg/ rGO exhibits effective amperometric detection of NADH at 0.15 V within a wide linear concentration range of 2– 2378 μM, a high sensitivity of 92.62 μA mM−1 cm−2, low detection limit of 0.2 μM, and long-term detection over 2500 s. Moreover, the as-constructed biosensors can achieve accurate NADH detection in human serum samples, indicating its promising application feasibility in fundamental and clinic research. Keywords Platinum-silver . Nanocomposite . Electrocatalyst . Electrochemical sensing
Introduction As an important coenzyme in the human body, NADH participates in hundreds of enzymatic reactions concerning over 300 dehydrogenases. The precise, convenient, and fast NADH quantification has attracted increasing research interests. Recently, the electrochemical method has been wildly developed for NADH detection, benefiting from the high accuracy, outstanding sensitivity, rapid response, good portability, as
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00604-020-04530-1) contains supplementary material, which is available to authorized users. * Caixia Xu [email protected] 1
Institute for Advanced Interdisciplinary Research (iAIR), School of Chemistry and Chemical Engineering, Collaborative Innovation Center of Technology and Equipment for Biological Diagnosis and Therapy in Universities of Shandong, University of Jinan, Jinan 250022, Shandong Province, China
2
Qilu University of Technology (Shandong Academy of Sciences), Jinan 250353, Shandong Province, China
well as convenient operation [1, 2]. However, the electrochemical quantification of NADH possesses several inherent weaknesses. The direct electron transfer of NADH at bare electrode is kinetically sluggish, highly irreversible, and accompanied by requiring large activation energy and high applied potentials [3]. Moreover, the electrochemical NADH oxidation is usually associated with side reacti
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