The effects of two iso-volume endurance training protocols on mitochondrial dysfunction in type 2 diabetic male mice
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RESEARCH ARTICLE
The effects of two iso-volume endurance training protocols on mitochondrial dysfunction in type 2 diabetic male mice Masoumeh Sadat Modaresi 1 & Mehrdad Fathei 1 & Seyed Reza Attarzadeh Hosseini 1 & Mohammad Mosaferi Ziaaldini 1 & Mohammad Reza Sadeghian Shahi 2 Received: 14 November 2019 / Revised: 18 July 2020 / Accepted: 7 August 2020 # Springer Nature Switzerland AG 2020
Abstract Purpose Type 2diabetes(T2D) is one of the more common diseases in the world and has been widely spread. One of the suggested mechanisms in development of T2D, is mitochondrial dysfunction. The purpose of this study is to compare the effects of two endurance training protocols with low and moderate intensity on biogenesis and mitochondrial function, in Diabetic mice induced by high fat diet and Streptozotocin(STZ). Methods 40 five week old mice divided to four groups including: health control (HC, n = 7), diabetic control (DC, n = 7), low endurance training (DLT, n = 7) and moderate endurance training (DMT, n = 7). DMT group ran at 5 m/min for an hour, 3 days a week on a treadmill, and DLT group ran at 3 m/min for an hour, 5 days a week on a treadmill for 8 weeks. Results The cytosolic content of PGC1α, Tfam and mitochondrial content of citrate synthase(Cs) and cytochrome c oxidase(Cox) in DC was significantly reduced compared to HC(P˂0.05). All of the parameters except for Cs in both DLT and DMT were increased compared to DC (P˂0.05), but there was no difference between them and the HC (P˃0.05). There was no difference in Cs enzyme between the DC and the DLT(P˃0.05), but it was significantly increased in the DMT(P˂0.05). There was a significantly difference between Cs enzyme in HC and DLT(P˂0.05), but there wasn’t any significant difference between HC and DMT(P˃0.05). Conclusions The results showed that in same volume condition, both endurance training protocols improved the proteins involved in biogenesis and mitochondrial function in T2D mice and there was no significant difference between them. Keywords Type 2 Diabetes . Mitochondrial dysfunction . PGC1α . Tfam . Citrate synthase . Cytochrome c oxidase
Abbreviations T2D Type 2 diabetes STZ Streptozotocin * Mehrdad Fathei [email protected] Masoumeh Sadat Modaresi [email protected] Seyed Reza Attarzadeh Hosseini [email protected] Mohammad Mosaferi Ziaaldini [email protected] Mohammad Reza Sadeghian Shahi [email protected] 1
Faculty of Sport Science, Ferdowsi University of Mashhad, Azadi sq, Mashhad, Iran
2
Department of Physical Education, Yazd University, Blvd University, Yazd, Iran
HC DC DLT DMT PGC1a Tfam Cs Cox NRF
Healthy control Diabetic control Diabetic low endurance training Diabetic moderate endurance training Peroxisome proliferator-activated receptor-coactivator-1α Transcription factor A citrate synthase cytochrome c oxidase Nuclear respiratory factor
Introduction It is estimated that there will be 600 million diabetic patients in 2040. According to the International Diabetes Foundation, every 6 s one person dies of diabetes [1
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