Protein Arginylation Methods and Protocols
This volume presents a comprehensive overview of all the existing methods on analysing protein arginylation, from the early methods utilizing crude protein preparations and whole-cell assays to the latest advanced methods involving recombinant protein tec
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Anna S. Kashina Editor
Protein Arginylation Methods and Protocols
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Protein Arginylation Methods and Protocols
Edited by
Anna S. Kashina Department of Animal Biology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA, USA
Editor Anna S. Kashina Department of Animal Biology School of Veterinary Medicine University of Pennsylvania Philadelphia, PA, USA
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-2934-4 ISBN 978-1-4939-2935-1 (eBook) DOI 10.1007/978-1-4939-2935-1 Library of Congress Control Number: 2015946756 Springer New York Heidelberg Dordrecht London © Springer Science+Business Media New York 2015 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Printed on acid-free paper Humana Press is a brand of Springer Springer Science+Business Media LLC New York is part of Springer Science+Business Media (www.springer.com)
Preface Posttranslational modifications are pivotal regulators of virtually every biological process and the major contributors to the complexity of functions in every physiological system. Arginylation—tRNA-mediated posttranslational addition of arginine to proteins—is a posttranslational modification of emerging importance that affects an estimated 25 % of the cellular proteome and is critical for multiple aspects of embryogenesis and the functioning of an adult organism. The intent of this book is to present a comprehensive overview of all the existing methods on analyzing protein arginylation, from the early methods utilizing crude protein preparations and whole-cell assays to the latest advanced methods involving recombinant protein techniques, antibodies, high precision mass spectrometry, and chemical probes. The book also includes essays from the founders of the f
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