Protein Folding, Misfolding, and Disease Methods and Protocols
Protein misfolding is a key feature of many disorders in humans, given that over twenty proteins are known to misfold and cause disease. In Protein Folding, Misfolding, and Disease: Methods and Protocols, experts in the field present a collection of
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Molecular Biology™
Series Editor John M. Walker School of Life Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Protein Folding, Misfolding, and Disease Methods and Protocols
Edited by
Andrew F. Hill Department of Biochemistry and Molecular Biology, Bio21 Molecular Science and Biotechnology Institute, University of Melbourne, Parkville, VIC, Australia
Kevin J. Barnham Department of Pathology, Bio21 Molecular Science and Biotechnology Institute, University of Melbourne, Parkville, VIC, Australia
Stephen P. Bottomley Department of Biochemistry and Molecular Biology, Monash University, Clayton, VIC, Australia
Roberto Cappai Department of Pathology, Bio21 Molecular Science and Biotechnology Institute, University of Melbourne, Parkville, VIC, Australia
Editors Andrew F. Hill Department of Biochemistry and Molecular Biology Bio21 Molecular Science and Biotechnology Institute University of Melbourne Parkville, VIC, Australia [email protected] Kevin J. Barnham Department of Pathology Bio21 Molecular Science and Biotechnology Institute University of Melbourne Parkville, VIC, Australia [email protected]
Stephen P. Bottomley Department of Biochemistry and Molecular Biology Monash University Clayton, VIC, Australia [email protected] Roberto Cappai Department of Pathology Bio21 Molecular Science and Biotechnology Institute University of Melbourne Parkville, VIC, Australia [email protected]
ISSN 1064-3745 e-ISSN 1940-6029 ISBN 978-1-60327-221-6 e-ISBN 978-1-60327-223-0 DOI 10.1007/978-1-60327-223-0 Springer New York Dordrecht Heidelberg London Library of Congress Control Number: 2011930672 © Springer Science+Business Media, LLC 2011 All rights reserved. This work may not be translated or copied in whole or in part without the written permission of the publisher (Humana Press, c/o Springer Science+Business Media, LLC, 233 Spring Street, New York, NY 10013, USA), except for brief excerpts in connection with reviews or scholarly analysis. Use in connection with any form of information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed is forbidden. The use in this publication of trade names, trademarks, service marks, and similar terms, even if they are not identified as such, is not to be taken as an expression of opinion as to whether or not they are subject to proprietary rights. Printed on acid-free paper Humana Press is part of Springer Science+Business Media (www.springer.com)
Preface Protein Folding, Misfolding, and Disease: Methods and Protocols brings together a collection of current methods for studying the analysis of protein folding and misfolding. Protein misfolding into structures, such as amyloid, is a key feature of many disorders of humans in which over 20 proteins are known to misfold and cause disease. The protocols in this volume cover strategies for expressing and refolding recombinant proteins wh
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