Biodegradation of petroleum refining industry oil sludge by microbial-assisted biocarrier matrix: process optimization u
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ORIGINAL PAPER
Biodegradation of petroleum refining industry oil sludge by microbial-assisted biocarrier matrix: process optimization using response surface methodology K. V. Swathi . R. Muneeswari . K. Ramani . G. Sekaran
Received: 15 July 2020 / Accepted: 29 September 2020 Ó Springer Nature B.V. 2020
Abstract Safe disposal of petroleum oil sludge generated from crude oil storage tank bottom is a major challenge for petroleum refineries across the globe. The presence of long chain hydrocarbons in petroleum oil sludge are known to have effects on the environment through bioaccumulation or biosorption. The present study was focused to develop a modified bioremediation process using hydrocarbonoclastic microbial-assisted biocarrier matrix (MABC) mediated through biosurfactants and biocatalysts for the efficient treatment of petroleum industrial oily sludge. The development of hydrocarbonoclastic microbialassisted biocarrier matrix was confirmed by scanning electron microscopy analysis. The biocatalysts such as lipase, laccase, esterase and biosurfactant produced by MABC system were found to be 40 U/mg, 18 U/mg, 36 U/mg and 220 mg/g of oil sludge respectively
using one variable at a time approach. Further, the response surface methodology was used to determine the optimum treatment conditions (Time, pH, Mass of biocarrier matrix and Amount of oil sludge) for the enhanced removal of total petroleum hydrocarbons (TPH) present in the oil sludge and TPH was degraded by 88.78% at Hydraulic Retention Time of 7 days. The biodegradation of oil sludge was confirmed using Gas Chromatography–Mass Spectrometry analysis. Keywords Petroleum refinery oil sludge TPH degradation Microbial-assisted biocarrier matrix Response surface methodology Biosurfactant Biocatalysts
Introduction Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10532-020-09916-9) contains supplementary material, which is available to authorized users. K. V. Swathi R. Muneeswari K. Ramani (&) Biomolecules and Biocatalysis Laboratory, Department of Biotechnology, SRM Institute of Science and Technology, Kancheepuram District, Kattankulathur, Tamil Nadu 603 203, India e-mail: [email protected] G. Sekaran SRM Institute of Science and Technology, Ramapuram, Tamil Nadu 600089, India
Petroleum oil refinery is one of the major industrial sector around the world contributing to world economy (Suganthi et al. 2018). The rapid increase in the human population has created a significant demand in the consumption of petroleum products to meet the transportation, energy, human health care and cosmetics requirements, which has led to a remarkable growth and development of petroleum oil refining industrial sector across the globe (Kuznetsova and Kravchenko 2020). More particularly, during the storage and transportation of crude oil, under the influence of gravity difference between the lighter and
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Biodegradation
heavier molecules present in the crude oil; hea
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