Challenges of Enzymes, Conidia and 6-Pentyl-alpha-pyrone Production from Solid-State-Fermentation of Agroindustrial Wast
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ORIGINAL PAPER
Challenges of Enzymes, Conidia and 6‑Pentyl‑alpha‑pyrone Production from Solid‑State‑Fermentation of Agroindustrial Wastes Using Experimental Design and T. asperellum Strains Rayhane Hamrouni1,2 · Magalie Claeys‑Bruno1 · Josiane Molinet1 · Ahmed Masmoudi2 · Sevastianos Roussos1 · Nathalie Dupuy1 Received: 26 March 2019 / Accepted: 25 November 2019 © Springer Nature B.V. 2019
Abstract In a context of growing awareness regarding environmental protection, biomass valorization is gaining a lot of attention. The byproducts volumes generated by agro-industry are massive and, left to decay, can constitute environmental pollutions. Use of agro-industrial wastes and solid-state fermentation (SSF) technology offers advantages to produce value-added products such as antibiotics, pigments, aromas and enzymes of industrial interest like cellulases, chitinases, amylases, etc. Several studies have already demonstrated the advantages of SSF for the production of fungal metabolites, yet the optimal conditions for metabolites production strongly depend on the culture conditions and microbial strain utilized. Therefore, the aim of this study was to improve the conidia, lytic enzymes (cellulase, lipase and amylase), and antifungal—6-pentylalpha-pyrones (6-PP)—production by three Trichoderma asperellum strains cultivated using SSF. Designs of experiments have been achieved in order to identify influential factors on 6-PP, conidia and enzymes (cellulase, lipase, and amylase) production by the fungal culture. A significantly enzymes activities, conidiation and 6-PP production were observed on mix of substrates: vine shoots, potatoes flour, jatropha, olive pomace and olive oil on high carbon/nitrogen ratio 37 which was used by T. asperellum TV104 as a source of nutrients and also as a matrix. Graphic Abstract
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Waste and Biomass Valorization
Keywords Solid-state-fermentation · Trichoderma asperellum · Optimization · Agroindustrial wastes · Experimental design
Statement of Novelty The development of sustainable solutions to exploit byproduct is necessary to solve the environmental problems. They are rich in nutrients can serve as a substrate for the production of interested compounds as antifungal ones. In this study various byproduct were valorized to formulate biopesticides by the way of solid state fermentation (SSF) and Trichoderma asperellum fungi. For the optimization of antifungal properties, Hadamard design was performed and various byproduct mixture. The final formulation allows to obtain high content in conidia, enzyme and 6-pentyl-alphapyrone (6-PP). This work presents two advantages: The valorization of agroindustrials wastes having an impact on the ecology of the world, and the production of interested compounds.
Introduction Commonly agricultural produce important amount of byproducts whose main part are unused and cause environmental problems [1, 2]. In recent years, numerous studies have been published support
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