Characterization and expression pattern analysis of pheromone receptor-like genes in Winter Mushroom Flammulina filiform
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ORIGINAL PAPER
Characterization and expression pattern analysis of pheromone receptor‑like genes in Winter Mushroom Flammulina filiformis Li Meng1,2 · Tiansheng Chou2,3 · Siyuan Jiang2,3 · Li Wang1 · Mengjuan Zhu1 · Irum Mukhtar2 · Baogui Xie2 · Wei Wang1,2 Received: 8 January 2020 / Revised: 2 June 2020 / Accepted: 18 July 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Pheromone receptor-like genes (PRLGs) belong to the G protein-coupled receptors (GPCRs) family that interacts with biotic and abiotic stimulants and transmits signals to intracellular downstream pathways in eukaryotic cells. In this study, we investigated the structure and expressions patterns of PRLGs in Winter Mushroom Flammulina filiformis. Based on the alignment analysis, the structure of PRLGs was found conserved in F. filiformis strains expect few single-nucleotide polymorphism (SNP) sites. Six PRLGs were found at five different unlinked loci, scattered in the genomes of F. filiformis strains. These genes contain 2–5 introns; however, the introns were not found in the same relative positions regarding the encoded protein sequences in tested strains of F. filiformis. Three conserved motifs were identified in peptides structures of PRLGs, however, FfSte3.s6 contained only two types, suggests its difference in evolution and function. We have further analyzed the expression patterns of each PRLGs in different developmental stages of the fruiting body in F. filiformis by quantitative real-time polymerase chain reaction (qRT-PCR). The results exhibited expression variation of PRLGs at different developmental stages of the F. filiformis. Especially, FfSte3.s1 and FfSte3.s2 exhibited maximum expression level in mycelia stage. Other PRLGs exhibited high expression level in fruiting body stages. This study suggests that PRLGs could be vital genes involving in fruiting body development in F. filiformis. However, further studies could be performed to reveal their specific functional pathways in the fruiting body development. Keywords Flammulina filiformis · Fruiting body · PRLG · Expression pattern · G protein-coupled receptors
Introduction Communicated by Erko Stackebrandt. Li Meng and Tiansheng Chou Contributed equally to this work. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00203-020-01990-0) contains supplementary material, which is available to authorized users. * Wei Wang [email protected] 1
Shandong Provincial Key Laboratory of Agricultural Microbiology, College of Plant Protection, Shandong Agricultural University, Tai’an 271018, China
2
Mycological Research Center, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China
3
BGI Education Center, University of Chinese Academy of Sciences, Shenzhen 518083, China
Sexual reproduction is a complex and significant process in life cycle of fungi. Fruiting body formation and development of edible mushrooms that belong to macrofungi is controlled by sexual loci called mat
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