Composite Matrices Based on Copolyamide and Polypyrrole for Tissue Engineering
- PDF / 1,105,251 Bytes
- 6 Pages / 612 x 792 pts (letter) Page_size
- 86 Downloads / 218 Views
MER MATERIALS FOR BIOMEDICINE
Composite Matrices Based on Copolyamide and Polypyrrole for Tissue Engineering N. V. Smirnovaa,b,*, I. Yu. Sapurinaa,b, M. A. Shishova,b, K. A. Kolbea,b, E. M. Ivan’kovaa,b, V. V. Matrenichevb, and V. E. Yudina,b a
b
Peter the Great St. Petersburg Polytechnic University, St. Petersburg, 195251 Russia Institute of High-Molecular Compounds, Russian Academy of Sciences, St. Petersburg, 199004 Russia *e-mail: [email protected] Received February 4, 2020; revised February 4, 2020; accepted February 17, 2020
Abstract—We have demonstrated the possibility of application of electroconducting polymers for fabricating bioactive matrices for tissue engineering. Polypyrrole is most promising among such polymers in the context of its biomedical applications. Polypyrrole possesses a number of properties that make it an adequate basis for preparing “smart” bioactive materials. To obtain the best mechanical properties of composite matrices, we used aliphatic copolyamide. The matrices obtained from the copolyamide solution had the structure of thin films, as well as fibrous nonwoven mats prepared by electroformation. Copolyamide films were modified with polypyrrole using polymerization by oxidation with the formation of composite matrices. The samples obtained in this way exhibited service properties acceptable for applications and an electric conductivity level sufficient for cell technologies. In the in vitro experiments, the copolyamide- and polypyrrole-based matrices support the viability, adhesion, and proliferation of human dermal fibroblasts. DOI: 10.1134/S1063784220100217
INTRODUCTION Modern investigations have made it possible to use electroconducting polymers (EPs) in a wide range of biomedical technologies, including the development of drug delivery systems and designing of biosensor systems and biocompatible electrodes for in vivo and in vitro applications. The possibility of using EPs for fabricating bioactive matrices intended for tissue technologies and tissue engineering has also been demonstrated [1]. Among the materials selected for regenerative technologies, electroconducting polymer matrices possess optimal properties for in vivo and in vitro transfer of an electric signal to cells. It was shown that exogenous electrical stimuli influence on fundamental aspects of living activity and behavior of cells (adhesion, migration, and proliferation). The differentiation priorities of cells also change under the action of electrostimulation [2]. Apart from in cells that have been traditionally considered electrosensitive, the response of dermal fibroblasts to electrostimulation aimed at improving the healing of skin wounds and soft tissues was demonstrated for in vivo and in vitro systems and in some clinical tests [3]. In addition, it was shown that electric signals are also important at the tissue level; for example, the maintaining of a constant electric potential between the external and internal skin layers is an important condition for sustaining its homeostasis. In the case of tra
Data Loading...
