Coronary corium, a new source of equine mesenchymal stromal cells
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ORIGINAL ARTICLE
Coronary corium, a new source of equine mesenchymal stromal cells Luiza Lopes da Silva 1 & Maiele Dornelles Silveira 2,3 & Carla Augusta Sassi da Costa Garcia 1 & Patrícia Bencke Grudzinski 4 & Charles Ferreira Martins 1 & Nance Beyer Nardi 2,3,4 Received: 15 October 2019 / Accepted: 20 February 2020 # Springer Nature B.V. 2020
Abstract Mesenchymal stromal cells (MSCs) have attracted great attention for therapeutic applications. Since cells derived from different tissues have different properties, using the right tissue source may impact their efficiency in regenerative medicine. This study describes for the first time the isolation and characterization of MSCs derived from the equine coronary corium, which may be useful for treating diseases such as laminitis. Seven coronary corium samples were used for isolation of cells (ccMSCs). Adherent cells were characterized for morphology, immunophenotype, proliferation and differentiation potential, in vitro migration and colony-forming capacity. The cells displayed the characteristic fibroblastoid morphology, with population doubling time increasing until passage 7 and reaching a plateau in passage 10. Cells were negative for CD14 and CD45, and positive for CD73 and CD90. ccMSCs showed chondrogenic and osteogenic, but not adipogenic differentiation, and migrated with nearly total closing of the empty area in 48 h, in the scratch assay. The clonogenic potential was in average 18% to 23%. This study describes for the first time the establishment of mesenchymal stromal cell cultures from the equine coronary corium. The results are similar to MSCs isolated from many other equine tissues, except for restricted differentiation potential. As coronary corium stem cell regulation may contribute to the pathogenesis of equine chronic laminitis, the use of ccMSCs in cell therapy for this significantly debilitating disease should be further investigated. Keywords Mesenchymal stromal cells . Horse . Coronary corium . Laminitis
Introduction Mesenchymal stem or stromal cells (MSCs) are multipotent progenitor cells that have attracted great attention in recent years for therapeutic applications. Initially described in the 1970s (Friedenstein et al. 1970), the cells received their current name 20 years later, in reference to adherent, cultureLuiza Lopes da Silva and Maiele Dornelles Silveira contributed equally to this work. * Nance Beyer Nardi [email protected] 1
Veterinary School, Universidade Federal de Pelotas, Campus Universitário, Pelotas, RS 96010900, Brazil
2
Laboratory of Stem Cells and Tissue Engineering, Universidade Luterana do Brasil, Canoas, RS 92425-900, Brazil
3
CellMed Medicina Regenerativa e Consultoria Científica, Porto Alegre, RS 90619-900, Brazil
4
Institute of Cardiology of Rio Grande do Sul, Fundação Universitária de Cardiologia, Av Princesa Isabel 395, Porto Alegre, RS 90040-371, Brazil
expanded bone marrow cells (Caplan 1991). MSCs have been extensively investigated for their biological properties and regenerative potential. Due to t
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