Dendritic Cell Protocols
Given the vital importance of immune system research, the gathering of clear, consistent, and informative protocols involving the study of dendritic cells is paramount. Bringing the popular first edition fully up to date, Dendritic Cell Protocols, Second
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MO L E C U L A R BI O L O G Y
Series Editor John M. Walker School of Life Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For other titles published in this series, go to www.springer.com/series/7651
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Dendritic Cell Protocols Second Edition
Edited by
Shalin H. Naik The Netherlands Cancer Institute, Amsterdam, The Netherlands
Editor Shalin H. Naik Quellijnstraat 25A 1072 XN Amsterdam Netherlands [email protected]
ISSN 1064-3745 e-ISSN 1940-6029 ISBN 978-1-60761-420-3 e-ISBN 978-1-60761-421-0 DOI 10.1007/978-1-60761-421-0 Library of Congress Control Number: 2009939693 © Humana Press, a part of Springer Science+Business Media, LLC 2010 All rights reserved. This work may not be translated or copied in whole or in part without the written permission of the publisher (Humana Press, c/o Springer Science+Business Media, LLC, 233 Spring Street, New York, NY 10013, USA), except for brief excerpts in connection with reviews or scholarly analysis. Use in connection with any form of information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed is forbidden. The use in this publication of trade names, trademarks, service marks, and similar terms, even if they are not identified as such, is not to be taken as an expression of opinion as to whether or not they are subject to proprietary rights. Printed on acid-free paper springer.com
Foreword Research on dendritic cells (DC) has certainly come of age. The initial recognition of DC by Steinman and Cohn was followed by a long lag before the immunological community was convinced that these relatively rare cells were special. It is now accepted that these antigen-presenting cells are essential for the initiation of primary T-cell responses as well as for the maintenance of self-tolerance. When improved methods of DC isolation and analysis revealed multiple DC subsets, there was an understandable reluctance to accept this level of complexity. This has changed to an enthusiastic investigation of a network of DC subtypes differing in location and migration routes, in their patterns of microbial recognition receptors, in whether they process antigen for MHC class I or MHC class II presentation, and in the cytokines and chemokines they produce. The initially confusing impression that all haematological pathways might lead to DC is changing as intermediate precursors in steady-state DC development are being isolated. New culture systems for producing the different DC subtypes have been developed to supplement the earlier culture procedure for generating inflammatory-type DC from monocytes. This volume usefully details many of the basic techniques now used in this modern era of DC research. However, these methods are not only a way of duplicating past experiments; they must help to lead us to the future. Although there is still much to be learnt from our steady-state laboratory mouse models, especially on the mucosal immune system, there are already some clear shif
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