Dendritic Cell Protocols
The third edition of this volume is aimed at providing both beginners and more experienced researchers a choice of methods to isolate and analyze dendritic cells(DC). An introductory review provides an overview of recent advances in the characterization o
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Elodie Segura Nobuyuki Onai Editors
Dendritic Cell Protocols Third Edition
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Dendritic Cell Protocols Third Edition
Edited by
Elodie Segura Pavillon Pasteur, Institut Curie INSERM U932, Paris, France
Nobuyuki Onai Department of Biodefense, Tokyo Medical and Dental University, Tokyo, Japan
Editors Elodie Segura Pavillon Pasteur Institut Curie INSERM U932 Paris, France
Nobuyuki Onai Department of Biodefense Tokyo Medical and Dental University Tokyo, Japan
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-3604-5 ISBN 978-1-4939-3606-9 (eBook) DOI 10.1007/978-1-4939-3606-9 Library of Congress Control Number: 2016939414 © Springer Science+Business Media New York 2016 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Printed on acid-free paper This Humana Press imprint is published by Springer Nature The registered company is Springer Science+Business Media LLC New York
Preface Dendritic cells are fascinating cells but can be challenging to work with. In this new edition of Dendritic Cell Protocols, we aimed at complementing the previous edition in order to provide both beginners and more experienced researchers a choice of methods to isolate and analyze dendritic cells. An introductory review provides an overview of recent advances in the characterization of DC subsets in mouse and human. Dendritic cells are rare, fragile, and their isolation is often a tedious procedure. For those who wish to generate dendritic cells in vitro, two chapters provide methods to culture human and mouse dendritic cells. Several chapters are devoted to protocols for the isolation of dendritic cells from various organs and tissues (lymphoid organs, intestine, skin, lung, liver), both in mouse and human. In addition, a chapter describes th
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